Establishment of regeneration of Actinidia arguta ‘Tianyuanhong’
Author(s): LIN Miao-Miao, FANG Jin-Bao?, QI Xiu-Juan, CHEN Jin-Yong, GU Hong, ZHANG Wei-Yuan, SUN Lei-Ming
Pages: 1358-1362,1302
Year: 2016 Issue: 11
Journal: Guihaia
Keyword: all-red Actinidia arguta; tissue culture; callus; multiple shoot clump;
Abstract: ‘Tianyuanhong’ was a new Actinidia arguta cultivar, with all red pericarp and pulp. In order to establish the rapid and efficient propagation system in vitro in‘Tianyuanhong’ , we used stem with axillary bud, young leaf and leaf stem of ‘Tianyuanhong’ as the explants, and used tissue culture methods to study explant types and the best plant growth regulator substance combination in this cultivar. The result showed that stem with axillary bud that sprout in early spring was the best explants, and MS+6-BA 0.5 mg·L-1+IBA 1.0 mg·L-1 was the suitable plant growth substances combination;leaf stem was the better experimental material than leaf to induce callus, MS+ZT 0.5 mg·L-1 was the opti-mizing plant growth substances combination;the best plant growth substances combination in adventitious bud propaga-tion was MS+ZT 1.0 mg·L-1;and 6-BA could have the same effect in adventitious bud propagation, and could replaceZT for adventitious bud propagation in production, MS+6-BA 2.0 mg·L-1+IBA 0.5 mg·L-1 was well;1/2 MS+NAA 0.2 mg·L-1 was the best plant growth substances combination for rooting. After the seedlings rooted, transplanted the seed-lings to the substrate, the seedlings could reach the 98% survival rate in the substrate with the ratio of perlite ∶ peat soil ∶ sand=1 ∶ 1 ∶ 1. Through this study, the in vitro regeneration system was established in all-red A. arguta‘Tianyuanhong’ , which provides a good technical support for seedling micropropagation and factory nursery, at the same time, the established regeneration system provides the basis for the research of genetic transformation in all-red A.arguta.
Pages: 1358-1362,1302
Year: 2016 Issue: 11
Journal: Guihaia
Keyword: all-red Actinidia arguta; tissue culture; callus; multiple shoot clump;
Abstract: ‘Tianyuanhong’ was a new Actinidia arguta cultivar, with all red pericarp and pulp. In order to establish the rapid and efficient propagation system in vitro in‘Tianyuanhong’ , we used stem with axillary bud, young leaf and leaf stem of ‘Tianyuanhong’ as the explants, and used tissue culture methods to study explant types and the best plant growth regulator substance combination in this cultivar. The result showed that stem with axillary bud that sprout in early spring was the best explants, and MS+6-BA 0.5 mg·L-1+IBA 1.0 mg·L-1 was the suitable plant growth substances combination;leaf stem was the better experimental material than leaf to induce callus, MS+ZT 0.5 mg·L-1 was the opti-mizing plant growth substances combination;the best plant growth substances combination in adventitious bud propaga-tion was MS+ZT 1.0 mg·L-1;and 6-BA could have the same effect in adventitious bud propagation, and could replaceZT for adventitious bud propagation in production, MS+6-BA 2.0 mg·L-1+IBA 0.5 mg·L-1 was well;1/2 MS+NAA 0.2 mg·L-1 was the best plant growth substances combination for rooting. After the seedlings rooted, transplanted the seed-lings to the substrate, the seedlings could reach the 98% survival rate in the substrate with the ratio of perlite ∶ peat soil ∶ sand=1 ∶ 1 ∶ 1. Through this study, the in vitro regeneration system was established in all-red A. arguta‘Tianyuanhong’ , which provides a good technical support for seedling micropropagation and factory nursery, at the same time, the established regeneration system provides the basis for the research of genetic transformation in all-red A.arguta.
Related Articles