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MECHANISM OF GENISTEIN INHIBITION OF HUMAN CYTOMEGALOVIRUS INFECTION IN HUMAN ASTROCYTES
Author(s): 
Pages: 260-264
Year: Issue:  3
Journal: Acta Aacademiae Medical Qingdao Universitatis

Keyword:  cytomegalovirusmicrogliagenisteinmicroRNAsimmunoprecipitation;
Abstract: Objective To explore the effectiveness and its mechanism of Genistein(Gen)in inhibition of human cytomegalovirus(HCMV)infection in human astrocytes(AS). Methods Five groups were set in this study:blank control group(group C),Gen-control group(group G),HCMV group(group V),HCMV+Gen group(group V+G)and Gen+HCMV group(group G+V).CCK-8assay was used to screen out the appropriate concentration of Gen.RT-qPCR was used to detect the expression changes of viral encoding gene IE1 and IE2protein,host’s hsa-miR-200 miRNA cluster and viral miR-UL112.Chromatin immunoprecipitation assay(ChIP)was used to detect the changes of histone H3 acetylation binding major immediate early promoter(MIEP). Results 10μmol/L of Gen was screened out to be used as the experimental drug concentration.Compared with group V,the expressions of IE1 and IE2genes down-regulated(F=14.790-126.000,P<0.05),and in some time periods,the expressions of hsa-miR-200 miRNA cluster and miR-UL112up-regulated(F=3.041-4 638.724,P<0.05),and the acetylation of histone H3 binding to viral MIEP declined(F=18.184-32.848,P<0.05). Conclusion Genistein can,to some extent,inhibit the abnormal proliferation of human astrocytes caused by HCMV infection.Its mechanism is likely that Genistein can inhibit the expressions of IE1 and IE2gene to a certain degree and the acetylation of histone H3 binding to viral MIEP and partly up-regulate the expressions of host’s hsa-miR-200 miRNA cluster and viral miR-UL112.
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