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EFFECT OF LOW-POWER LASER IRRADIATION ON THE EXPRESSION OF p-ERK1/2 IN PERIODONTAL LIGAMENT CELLS AT ORTHODONTIC TENSION SIDE IN RATS
Author(s): 
Pages: 315-318
Year: Issue:  3
Journal: Acta Aacademiae Medical Qingdao Universitatis

Keyword:  laser therapylow-levelperiodontal ligamentosteogenesissignal pathwayratsWistar;
Abstract: Objective To investigate the effects of low-power laser irradiation on extracellular regulated protein kinases(p-ERK1/2)expression in periodontal ligament cells at the tension side of orthodontic teeth in rats and evaluate its effect on osteogenic differentiation of periodontal ligament cells. Methods Forty healthy male Wistar rats were recruited to create a modified orthodontic tooth movement model.Taking the two anterior teeth as a whole to be an anchorage,bilateral upper first molars were then mesially moved with 40 g of traction.The left or right side of the upper jaw was divided into group A(control group)and group B(laser irradiated 5s)in random.The alveolar periphery of first molar in group B was irradiated with weak laser(650nm,100mW).The rats were sacrificed on days 1,3,5,7and 14,respectively,after irradiation.Bone tissue slices containing maxillary first molar were prepared and immunohistostaining done.Expressions of p-ERK1/2,osteogenic differentiation-related protein Runx2 and alkaline phosphatase(ALP)in periodontal ligament cells on tension side were detected,their correlation was analyzed.Results The ALP mainly expressed in cytoplasm and interstitial substance of peridental membrane,on days 1,3and 5after radiation,its expression in group B was higher than that in group A,the difference was significant(F=121.02-716.60,P<0.01).Runx2 was prominently expressed in nucleus of the periodontal ligament cells,on days 1and 3,the difference in its expression between the two groups was not significant,but on days 5,7and 14,its expression in group B was higher than that in group A(F=19.43-43.42,P<0.01).p-ERK1/2was expressed in the nucleus and cytoplasm of periodontal ligament cells,on day 5after radiation,its expression in group B was the most notable;on day 7,its expression in group A increased,and that in group B decreased;on day 5,its expression in B was higher than that in group A,the difference was significant(F=38.99,P<0.01).Correlation analysis showed that the expressions of ALP and Runx2 were positively correlated with p-ERK1/2(r=0.399,0.799;P<0.01).Conclusion Weak laser exposure can obviously raise the expressions of p-ERK1/2,ALP and Runx2 in periodontal ligament cells on the tension side of orthodontic tooth in rats.The p-ERK1/2signal pathway may be involved in weak laser irradiation and promote osteogenic differentiation and bone remodeling of the periodontal ligament cells on the tension side of orthodontic teeth.
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