Analysis of Bacterial Community Structure in Vineyard Soil by RT-PCR and High Throughput Sequencing
Author(s): WANG Xiaowen, HONG Zhenhan, LIU Anrui, LUO Lixin
Pages: 28-33,36
Year: 2016 Issue: 11
Journal: Liquor-Making Science & Technology
Keyword: vineyard soil; rhizosphere soil; bacterial diversity; high throughput sequencing; quantitative PCR (Q-PCR);
Abstract: Soil bacteria have important ecological functions and play a very important role in vineyard ecological system. In this study, we per-formed quantitative PCR (Q-PCR) and Illumina Miseq high-throughput sequencing to compare the basic physiochemical parameters, enzymat-ic activities and bacterial groups between the rhizosphere and non-rhizosphere soil from a vineyard from Zhenyuan, Guizhou. Q-PCR results in-dicated that, the bacterial 16S rRNA gene copy numbers in the rhizosphere soils ranged from 1.38×108 to 1.17×109 copies/g, higher than that in non-rhizosphere soil. Illumina Miseq high-throughput sequencing results indicated that, the bacterial communities in rhizosphere vineyard soil mainly included Protecbacteria, Actinbacteria, Acidobacteria, Firmicute, Chloroflexi, Cyanobacteria, Crenarchoeo,Bacteroidetes,Gemmatimon-adete, and Nitrospirae, among them, the dominant bacterial taxa members were Proteobacteria and Actinobacteria which accounted for 53.33%~67.28%of total abundance. At genus level, Lactococcus, Arthrobacter, Candidatus Nitrososphaera, Nitrososphaera, Thermomonas, Phycicoccus, Pantoea, Bacillus, Escherichia, Sphingomonas, Lysobacter were the dominant bacteria genera in rhizosphere vineyard soil.α-di-versity analysis showed that, the bacterial community diversity of rhizosphere soil was similar in different site but were lower than that of the non-rhizosphere soil. Redundancy analysis showed that, there were certain correlations between bacterial communities and nutrients and en-zyme activity in vineyard soil..
Pages: 28-33,36
Year: 2016 Issue: 11
Journal: Liquor-Making Science & Technology
Keyword: vineyard soil; rhizosphere soil; bacterial diversity; high throughput sequencing; quantitative PCR (Q-PCR);
Abstract: Soil bacteria have important ecological functions and play a very important role in vineyard ecological system. In this study, we per-formed quantitative PCR (Q-PCR) and Illumina Miseq high-throughput sequencing to compare the basic physiochemical parameters, enzymat-ic activities and bacterial groups between the rhizosphere and non-rhizosphere soil from a vineyard from Zhenyuan, Guizhou. Q-PCR results in-dicated that, the bacterial 16S rRNA gene copy numbers in the rhizosphere soils ranged from 1.38×108 to 1.17×109 copies/g, higher than that in non-rhizosphere soil. Illumina Miseq high-throughput sequencing results indicated that, the bacterial communities in rhizosphere vineyard soil mainly included Protecbacteria, Actinbacteria, Acidobacteria, Firmicute, Chloroflexi, Cyanobacteria, Crenarchoeo,Bacteroidetes,Gemmatimon-adete, and Nitrospirae, among them, the dominant bacterial taxa members were Proteobacteria and Actinobacteria which accounted for 53.33%~67.28%of total abundance. At genus level, Lactococcus, Arthrobacter, Candidatus Nitrososphaera, Nitrososphaera, Thermomonas, Phycicoccus, Pantoea, Bacillus, Escherichia, Sphingomonas, Lysobacter were the dominant bacteria genera in rhizosphere vineyard soil.α-di-versity analysis showed that, the bacterial community diversity of rhizosphere soil was similar in different site but were lower than that of the non-rhizosphere soil. Redundancy analysis showed that, there were certain correlations between bacterial communities and nutrients and en-zyme activity in vineyard soil..
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