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chi gou fen zhi gan jun zai kang suan ran se shi yan jiao xue zhong de ying yong
Author(s): 
Pages: 21-22
Year: Issue:  10
Journal: Experiment Teaching and Apparatus

Keyword:  耻垢分枝杆菌卡介苗抗酸染色;
Abstract: 从耻垢分枝杆菌的培养特性和染色特性,可以判断其是否可替代卡介苗成为实验教学中抗酸染色法的菌种。耻垢分枝杆菌接种脑心浸液固体培养基和液体培养基培养,分别于接种后24 h,48 h,72 h和96 h对培养物进行抗酸染色。耻垢分枝杆菌在脑心浸液固体培养基中培养24 h,48 h,72 h和96 h都能呈现较好的抗酸染色效果,且在培养48 h染色效果最佳,而在液体培养基中培养48 h,72 h和96 h能观察到抗酸染色阳性结果,但细菌形态逐渐变短小,在染色时间上,6 min染色效果优于4 min染色。实验证明,耻垢分枝杆菌可替代卡介苗作为抗酸染色的菌种使用,充足的染色时间能保证较好的染色效果。
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