Inhibitory Effects of C-phycocyanin Combined with All-transretinoic Acid on the Growth of HeLa Cells
Author(s): YANG Fan, LI Bing, XU Ying-jie, LV Cong-yi, CHU Xian-ming, NIE Shu-min, Department of Biology, Medical College of Qingdao University, Department of Cardiology, the Affiliated Hospital of Medical College of Qingdao University, Department of Special Inspection, the Affiliated Hospital of Medical College of Qingdao University
Pages: 3248-3254
Year: 2014 Issue: 17
Journal: Biomagnetism
Keyword: All-transretinoic acid; C-phycocyanin; HeLa cells; Apoptosis mechanism;
Abstract: Objective: We focused on studying the effects of All-transretinoic acid(ATRA), C-phycocyanin(C-PC) or ATRA +C-PC on HeLa cell growth, cell cycle distribution and apoptosis. Methods: MTT assay was adopted to determine the effects of C-PC and/or ATRA on the growth of HeLa cells. The expressions of CDK-4 and Bcl-2 were determined by in situ hybridization and immunohistochemistry staining. TUNEL assay was used to analyze the cell apoptosis. Results: Both C-PC and ATRA could inhibit the growth of HeLa cells. And these two drugs showed synergistically inhibitory effects. The dosage of ATRA was reduced when used with C-PC. ATRA, combined with C-PC, was more efficient at inducing cell cycle arrest by decreasing CDK-4 expression. The combination of the two drugs could lead to down-regulation of Bcl-2 expression and thereby cell apoptosis. Conclusion: By combined with C-PC,ATRA can be used at lower dosage with reduced toxicity. C-PC + ATRA combination might exert the inhibitory effects on cell growth by inducing cell cycle arrest and cell apoptosis.
Pages: 3248-3254
Year: 2014 Issue: 17
Journal: Biomagnetism
Keyword: All-transretinoic acid; C-phycocyanin; HeLa cells; Apoptosis mechanism;
Abstract: Objective: We focused on studying the effects of All-transretinoic acid(ATRA), C-phycocyanin(C-PC) or ATRA +C-PC on HeLa cell growth, cell cycle distribution and apoptosis. Methods: MTT assay was adopted to determine the effects of C-PC and/or ATRA on the growth of HeLa cells. The expressions of CDK-4 and Bcl-2 were determined by in situ hybridization and immunohistochemistry staining. TUNEL assay was used to analyze the cell apoptosis. Results: Both C-PC and ATRA could inhibit the growth of HeLa cells. And these two drugs showed synergistically inhibitory effects. The dosage of ATRA was reduced when used with C-PC. ATRA, combined with C-PC, was more efficient at inducing cell cycle arrest by decreasing CDK-4 expression. The combination of the two drugs could lead to down-regulation of Bcl-2 expression and thereby cell apoptosis. Conclusion: By combined with C-PC,ATRA can be used at lower dosage with reduced toxicity. C-PC + ATRA combination might exert the inhibitory effects on cell growth by inducing cell cycle arrest and cell apoptosis.
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