Author(s): LIULin, LIShengmao, YUANBin, SHUBo, ZHANGShipeng
Pages: 18-21
Year: 2016 Issue:  17
Journal: Chinese Journal of Ethnomedicine and Ethnopharmacy
Keyword:  Pueraria lobata; Pueraria thomsonii; Extracts;
Abstract: Objective Comparative studies on scavenging activity of DPPH free radicals of Pueraria lobata and Pueraria thomso-nii.Methods Pueraria lobata and Pueraria thomsonii were extracted by ethanol,acetone,ethyl acetate,chloroform and petroleum e-ther,and the antioxidant activity was evaluated by scavenging activity of DPPH free radicals.Results The IC50 of the ethanol,ace-tone and ethyl acetate extracts of Pueraria lobata was 2.6334,17.5689,42.1171mg/mL (crude drug)respectively,and the rate of DPPH free radicals scavenging of chloroform and petroleum ether extracts at the concentration of 200mg/mL (crude drug) was 18.03%,9.48% respectively.The IC50 of the ethanol extracts of Pueraria thomsonii was 12.4629mg/mL (crude drug),and the rate of DPPH free radicals scavenging of acetone,ethyl acetate,chloroform and petroleum ether extracts at the concentration of 200mg/mL (crude drug)was 45.97%,15.03%,10.82%and 7.65%respectively.Conclusion All the extracts of Pueraria lobata and Pueraria thomsonii exhibited scavenging activity of DPPH free radicals,and the sequence of the activity was of ethanol extracts>acetone extracts>ethyl acetate extracts>chloroform extracts>petroleum ether extracts,and the scavenging activity of DPPH free radi-cals of Pueraria lobata was better than Pueraria thomsonii.