The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit later.
We apologize for any inconvenience caused
Login  | Sign Up  |  Oriprobe Inc. Feed
China/Asia On Demand
Journal Articles
Laws/Policies/Regulations
Companies/Products
Feasibility study on the differentiation of human adipose-derived mesenchymal stem cells into islet-like cells and in vitro construction of tissue-engineering islets
Author(s): 
Pages: 509-512+597
Year: Issue:  7
Journal: Journal of Logistics University of PAPF(Medical Sciences)

Keyword:  Adipose-derived mesenchymal stem cellsDifferentiationIslet-like cellsDecelluarized rat pancreasIslet tissue engineering;
Abstract: 【Objective】To study the feasibility of constructing tissue-engineering islets in vitro by seeding islet-like cells differentiated from adipose-derived mesenchymal stem cells(ADMSCs) in biological scaffolds of decellularized rat pancreas.【Methods】The human adipose tissues were collected by liposuction. Then ADMSCs were isolated and cultured. The cell phenotypes were identified by flow cytometry. The islet-like cells were differentiated from ADMSCs by inducers. The cell differentiation markers and insulin secretion were detected by dithizone staining, RT-PCR and ELISA. The decellularized rat pancreas was made by repeated freeze-thawing method combined with nuclease digestion. Tissue-engineering islets were constructed by seeding islet-like cells differentiated from ADMSCs in decellularized scaffolds. Morphological characteristics of tissue-engineering islets were observed under fluorescence microscopes. The proliferation of tissue-engineering islets was analyzed by detecting the changes of genomic DNA content.【Results】The undifferentiated ADMSCs appeared as adherent long spindle-shaped cells, and they were positive for CD73, CD90 and CD105, but negative for CD31, CD34 and CD45.The cells gradually became round and formed clusters after the induction. Dithizone staining showed the clusters were orange red. RT-PCR detection indicated that the expression of insulin gene was positive. The cells possessed the function of insulin secretion. Islet-like cells grew well on matrix surface equably and still had the capability of insulin secretion. However, the ability of proliferation decreased.【Conclusion】Islet-like cells differentiated from ADMSCs can proliferate well on the decellularized scaffolds. Therefore it is feasible to preliminarily construct tissue-engineering islets in vitro.
Related Articles
loading...