EFFECTS OF FREQUENCY DEPENDENT ELECTRICAL STIMULATION ON STOMACHACHE AND C-FOS EXPRESSION IN RATS
Author(s): LI Ying, ZHANG Chao, WANG Xin, FEI Yan, ZHOU Qiang-Qiang, CHEN Song-Song, ZHANG Li-CaiΔ
Pages: 591-597,602
Year: 2016 Issue: 8
Journal: Chinese Journal of Pain Medicine
Keyword: Stomachache; Refferred area; Electrical stimulation;
Abstract: Objective: To provide the experimental basis for the simple and noninvasive electric stimulation to relieve stomachache and the mechanism of stomachache referred pain.Methods: (1) In this experiment, the SD rats were given intragastric administration with 10% formalin, to establish the stomachache model. Evans blue was injected into SD rats' tails vein, the "leakage spots" in the body surface area was regarded as the stomachache referred area;(2) Applying electrical stimulation in the stomachache referred area with different frequencies such as 2 Hz、100 Hz and 2/100 Hz using HANS, afterwards, we observed SD rats' changes of pain-related behavior at some specific points in time, the expression of c-fos of referred areas were detected further by immunohistochemical and western blot, at last, we analyzed the correlation of various factors.Results: The leakage spots of Evans blue were not detected at normal rats. However, obvious spots were seen at shoulder blades of rats suffered from stomachache. After electrical stimulation at referred area, the pain scores and c-fos expression were the lowest in 2/100 Hz group, compared with other groups (P< 0.001).Conclusion: The rat's skin referred area of stomachache was located in shoulder blades. The 2/100 Hz electric stimulation at the referred area can relieve rats' stomachache. There are positive correlation between its effect and the expression of signaling molecules such as c-fos related to particular gastric motor and sensory neurons. This experiment provides the experimental basis for the simple and noninvasive electric stimulation to relieve stomachache and the mechanism of stomachache referred pain.
Pages: 591-597,602
Year: 2016 Issue: 8
Journal: Chinese Journal of Pain Medicine
Keyword: Stomachache; Refferred area; Electrical stimulation;
Abstract: Objective: To provide the experimental basis for the simple and noninvasive electric stimulation to relieve stomachache and the mechanism of stomachache referred pain.Methods: (1) In this experiment, the SD rats were given intragastric administration with 10% formalin, to establish the stomachache model. Evans blue was injected into SD rats' tails vein, the "leakage spots" in the body surface area was regarded as the stomachache referred area;(2) Applying electrical stimulation in the stomachache referred area with different frequencies such as 2 Hz、100 Hz and 2/100 Hz using HANS, afterwards, we observed SD rats' changes of pain-related behavior at some specific points in time, the expression of c-fos of referred areas were detected further by immunohistochemical and western blot, at last, we analyzed the correlation of various factors.Results: The leakage spots of Evans blue were not detected at normal rats. However, obvious spots were seen at shoulder blades of rats suffered from stomachache. After electrical stimulation at referred area, the pain scores and c-fos expression were the lowest in 2/100 Hz group, compared with other groups (P< 0.001).Conclusion: The rat's skin referred area of stomachache was located in shoulder blades. The 2/100 Hz electric stimulation at the referred area can relieve rats' stomachache. There are positive correlation between its effect and the expression of signaling molecules such as c-fos related to particular gastric motor and sensory neurons. This experiment provides the experimental basis for the simple and noninvasive electric stimulation to relieve stomachache and the mechanism of stomachache referred pain.
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