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Determination of Ferullic Acid in Duliang Pills by HPLC
Author(s): ZHANGYanqiu, WANGJing, YUANZimin, ZHANGZhenqiu
Pages: 15-
16
Year: 2016
Issue:
15
Journal: Chinese Journal of Ethnomedicine and Ethnopharmacy
Keyword: Duliang Pills; Ferullic acid; HPLC;
Abstract: Objective To establish HPLC for determining the content of ferullic acid in Duliang Pills.Method An HPLC method was developed.The chromatographic conditions were as follow:Diamonsil C18 column and methanol-0. 1%phosphoric acid (65∶35)as mobile phase,The column temperature was 25℃,detection wavelength at 321nm.Result The recovery range of ferullic acid was 0. 0144~0. 288μg,r=0. 9997.The average recovery rate was 98. 3%and RSD was 1. 02%.Conclusion The method is simple and accurate, with good repeatability,and can be used for determination of ferullic acid in Duliang Pills.
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