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Antifungal Activity of Pseudomonas choloeaphtis HL5-4 Against Tomato Gray Mold and Its Colonization Ability
Author(s): 
Pages: 1195-1202
Year: Issue:  6
Journal: Acta Horticulturae Sinica

Keyword:  Pseudomonas chlororaphistomato gray moldantifangal activitycolonization ability;
Abstract: 为探讨绿针假单胞菌HL5-4对番茄灰霉病的生防潜力,通过对峙培养和孢子萌发试验测定其对灰葡萄孢菌的抑制活性;进行离体试验和苗期试验测定其对番茄灰霉病的防治效果;采用平板稀释法测定其在番茄果实和叶片的定殖能力;并根据形态特征、生理生化特性及16S rDNA序列同源性对其进行鉴定.结果表明:HL5-4显著抑制灰葡萄孢菌的菌丝生长和分生孢子萌发,萌发抑制率为70.74%;在离体试验中,对番茄果实和叶片灰霉病的防治效果分别达到72.36%和59.85%,在盆栽试验中,对番茄苗期灰霉病的防治效果为62.88%;HL5-4在番茄果实和叶片保持一定的定殖密度,处理7d时能保持104和103 cfu· g-1以上;HL5-4菌株鉴定为绿针假单胞菌(Pseudomonas choloeaphtis).
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