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Optimal timing of autophagy occurrence induced by earle’s balanced salts solution in DLD -1, HCT -116,A2780,CHO,Hep G2 and SMMC7721 cancer cell lines
Author(s): 
Pages: 108-117
Year: Issue:  4
Journal: Acta Scientiarum Naturalium Universitatis Sunyatseni

Keyword:  autophage inductionEBSScancer cells;
Abstract: Autophagy has emerged as a key effector in tumorgenesis,but its precise function remains elusive.Au-tophagy flux is too low to be detected in normal culture conditions.Earle’s balanced salts solution (EBSS)is often used to induce autophage with two distinct advantages compared with other inducers:one is only needed to consid-erate the induction time,rather than the drug concentration;the other is that it exerts only starvation pressure by specifically simulating amino acids deprivation to cells without other undistinguishable cytotoxicity.Given the toler-ance threshold of outside stimulus differs in various types of tumor cells,in this study we carried out the autophage induction experiments with several time points in DLD -1 and HCT -116 colon cancer cell lines,A2780 and CHO ovarian cancer cell lines,Hep G2 and SMMC7721 hepatocellular carcinoma cell lines.Our western-blotting and Immunofluorescence staining results demonstrated that EBSS can efficiently induce autophagy in the assayed cells, precisely with 2 hours treatment in DLD -1,HCT -116,Hep G2 and CHO cells,4 hours in A2780 and 8 hours in SMMC7721 cells,respectively.Our results provide a reference for autophagy induction with EBSS in cancer cells, and would be useful to shorten the time-consuming optimizing process.
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