The interaction of quinoline-organogermanium sesquioxides with DNA
Author(s): SUI Beibei, ZHANG Lili, ZHAO Ying, GE Shun, SHANGGUAN GuoqiangΔ
Pages: 165-169,173
Year: 2016 Issue: 3
Journal: Journal of Jining Medical College
Keyword: Organogermanium sesquioxide; Interaction; Intercalation; Binding constant;
Abstract: Objective To study the interaction of two novel quinoline-organogermanium sesquioxides with calf thymus DNA(CT-DNA)and two synthetic oligonucleotides,d(AT)22 and d(GC)22,and reveal the anti-cancer mechanism of the organogermanium compounds. Methods The interaction was investigated by absorp-tion spectroscopy,DNA thermal denaturalization,viscosity,and fluorometric titration method. The binding con-stants were calculated from fluorescence-titration data by nonlinear least-squares analysis. Results The new compounds could interact with DNA by intercalation. The binding constants of the compounds with CT-DNA and the synthetic oligonucleotides were found to be on the order of 104-105 L/ mol. Conclusion DNA may be the primary effect target of the quinoline-organogermanium sesquioxides. These results provided the important information for the design and synthesis of new types of organogermanium compounds with stronger antican-cer activity.
Pages: 165-169,173
Year: 2016 Issue: 3
Journal: Journal of Jining Medical College
Keyword: Organogermanium sesquioxide; Interaction; Intercalation; Binding constant;
Abstract: Objective To study the interaction of two novel quinoline-organogermanium sesquioxides with calf thymus DNA(CT-DNA)and two synthetic oligonucleotides,d(AT)22 and d(GC)22,and reveal the anti-cancer mechanism of the organogermanium compounds. Methods The interaction was investigated by absorp-tion spectroscopy,DNA thermal denaturalization,viscosity,and fluorometric titration method. The binding con-stants were calculated from fluorescence-titration data by nonlinear least-squares analysis. Results The new compounds could interact with DNA by intercalation. The binding constants of the compounds with CT-DNA and the synthetic oligonucleotides were found to be on the order of 104-105 L/ mol. Conclusion DNA may be the primary effect target of the quinoline-organogermanium sesquioxides. These results provided the important information for the design and synthesis of new types of organogermanium compounds with stronger antican-cer activity.
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