Impacts of Astragalus Extract on the Alveolar Inflammation and Anti-Fibrosis Effects in Pulmonary Fibrosis Mice
Author(s): Xu Changjun, Fang Songwen, Dan Yong, Zhang Chuan, Xu Linlin, Liu Yang, Zhang Lili, Yang Changfu, Basic Medical College, Guiyang College of Traditional Chinese Medicine, The Nurse School, Guizhou Province People’s Hospital
Pages: 646-652
Year: 2016 Issue: 4
Journal: World Science and Technology-Modernization of Traditional Chinese Medicine
Keyword: Astragalusmembranaceus sugar; astragalus flavones; astragalus saponin; pulmonary fibrosis; inflammatory; TNF?α; TGF?β1;
Abstract: Astragalus was widely used in the TCM treatment of pulmonary inflammation in fibrosis. This study aimed to exam the therapeutic effects of flavonoids, polysaccharides and saponins in astragalus on pulmonary inflammation in bleomycin(BLM) induced fibrosis KM mice model. BLM were administered intratracheally in the model group and the medication group, while the same volume of normal saline in the control group. Mice were sacrificed after astragalus ingredients administration for 28 days. It was found that neutrophile granulocyte and macrophagocyte in bronchoalveolar lavage fluid were significantly increased in the model group(P < 0.05), which were reversed after administration of astragalus flavonoids. The same cell count results were showed both in Masson and HE staining(P < 0.05). And a positive correlation between inflammatory response and pulmonary fibrosis was presented in the study. However, there was no significant change after the treatments of astragalus polysaccharides, saponins and astragalisaponins plus flavonoids. Quantifing analysis of collagen was consistent with that of inflammation. Astragalus saponins and flavonoids both restrained early fibrosis nodules, while astragalus flavone was superior to the astragalus saponin group and the astragalus saponin plus flavone group. TNF?α, TGF?β1 were significantly increased in IHC and western blot results in the model group. Tragalus astragalus flavonoids reversed these trends, which surpassed other ingredients. In conclusion, astragalus flavone and astragalus saponin can inhibit pulmonary inflammation and fibrosis, which may be related to the antiinflammatory effects of TNF?α and TGF?β1.
Pages: 646-652
Year: 2016 Issue: 4
Journal: World Science and Technology-Modernization of Traditional Chinese Medicine
Keyword: Astragalusmembranaceus sugar; astragalus flavones; astragalus saponin; pulmonary fibrosis; inflammatory; TNF?α; TGF?β1;
Abstract: Astragalus was widely used in the TCM treatment of pulmonary inflammation in fibrosis. This study aimed to exam the therapeutic effects of flavonoids, polysaccharides and saponins in astragalus on pulmonary inflammation in bleomycin(BLM) induced fibrosis KM mice model. BLM were administered intratracheally in the model group and the medication group, while the same volume of normal saline in the control group. Mice were sacrificed after astragalus ingredients administration for 28 days. It was found that neutrophile granulocyte and macrophagocyte in bronchoalveolar lavage fluid were significantly increased in the model group(P < 0.05), which were reversed after administration of astragalus flavonoids. The same cell count results were showed both in Masson and HE staining(P < 0.05). And a positive correlation between inflammatory response and pulmonary fibrosis was presented in the study. However, there was no significant change after the treatments of astragalus polysaccharides, saponins and astragalisaponins plus flavonoids. Quantifing analysis of collagen was consistent with that of inflammation. Astragalus saponins and flavonoids both restrained early fibrosis nodules, while astragalus flavone was superior to the astragalus saponin group and the astragalus saponin plus flavone group. TNF?α, TGF?β1 were significantly increased in IHC and western blot results in the model group. Tragalus astragalus flavonoids reversed these trends, which surpassed other ingredients. In conclusion, astragalus flavone and astragalus saponin can inhibit pulmonary inflammation and fibrosis, which may be related to the antiinflammatory effects of TNF?α and TGF?β1.
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