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Effect of RAPA Combined with SAHA on Proliferation of Human Lung Adenocarcinoma A549 Cells after Radiotherapy
Author(s): 
Pages: 5-8
Year: Issue:  2
Journal: Acta Academiae Medicinae Jiangxi

Keyword:  rapamycinsuberoylanilide hydroxamic acidhuman lung adenocarcinoma A549 cellsradiotherapy sensitization;
Abstract: ABSTRACT:Objective To observe the effect of rapamycin(RAPA)combined with suberoylani-lide hydroxamic acid(SAHA)on the proliferation of human lung adenocarcinoma A549 cells after radiotherapy.Methods A549 cells were treated with RAPA(0,1,10,100,1000 nmol/L),SAHA (0,2.5,5,10 μmol·L-1 ),or their combination for 24 hours.The proliferation of cells was meas-ured by MTT assay.The combination index Q value was calculated to evaluate the combined effect of RAPA and SAHA.Moreover,the 10% inhibitory concentration(IC10)of the combina-tion of two drugs was calculated.Then,the survival fraction(SF)of A549 cells after radiotherapy and combined treatment with RAPA and SAHA at IC10 was detected by cell cloning assay.Re-sults Both RAPA and SAHA suppressed the proliferation of A549 cells in a dose dependent manner.Compared with single treatment with RAPA or SAHA,the suppression rate was signifi-cantly increased by their combination(P <0.05).In addition,the SF value in RAPA+SAHA+ra-diotherapy group(21.1 ± 7.0)% was markedly lower than that in radiotherapy group(67.8 ± 6.3)%,RAPA radiotherapy group(43.1±7.2)% or SAHA+radiotherapy group(58.5±1.2)%(P <0.05).Conclusion The IC10 of combination of RAPA and SAHA can enhance the sensitiza-tion of A549 cells to radiotherapy.%
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