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Homology Analysis of Vibrio Parahaemolyticus Isolates from Food Poisoning Outbreak Using Pulsed-Field Gel Electrophoresis
Pages: 32-34
Year: Issue:  2
Journal: Acta Academiae Medicinae Jiangxi

Keyword:  pulsed-field gel electrophoresisVibrio parahaemolyticushomology analysisfood poisoning;
Abstract: Objective To conduct the homology analysis of Vibrio parahaemolyticus strains iso-lated from a food poisoning outbreak using pulsed-field gel electrophoresis technology (PFGE). Methods Molecular subtyping of 7 Vibrio parahaemolyticus strains isolated from anal swabs of a patient with food poisoning was performed by PFGE after NotⅠdigestion.The DNA fingerprint maps of different Vibrio parahaemolyticus strains were analyzed by BioNumerics software.Results PFGE patterns of the 7 strains showed a 100% similarity.Conclusion PFGE is applicable to the homology analysis of isolates from food poisoning outbreak.
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