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The role of endophilin Ⅰ in Remifentanil-induced hyperalgesia in rats
Author(s): JIANG Wen-ting, YE Liu, BAI Xue, LI Yuan, GOU Chen-yu, CHEN Yuan, Research Center for Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University
Pages: 89-
94
Year: 2016
Issue:
2
Journal: Anatomy Research
Keyword: Remifentanil; Hyperalgesia; Endophilin Ⅰ; Hippocampus;
Abstract: Objective To explore the expression of Endophilin Ⅰ in the hippocampus of rats and its role during the development of hyperalgesia induced by the infusion of remifentanil. Methods 12 male SD rats were randomly divided into 2 groups,control group and remifentanil group(6 rats for each group). Hyperalgesia was induced by intravenous infusion of remifentanil for 2 hours.And nociception was assessed by Von Frey test and Hargreaves methods from 2 hours to 72 hours after remifentanil infusion. The changes of expression of Endophilin Ⅰ,NR1 and μ opioid receptor in hippocampus was observed in immunofluorescence and western blot analysis. Results Intravenous infusion of remifentanil led to significant hyperalgesia. Immunofluorescence and Western blot results showed that the expression of Endophilin Ⅰ was significantly upregulated(P<0.01)in the hippocampus of rats of hyperalgesia induced by remifentanil infusion,and NR1 was also upregulated(P<0.01),while μopioid receptor were downregulated(P<0.01).Conclusion Intravenous infusion of remifentanil led to significant hyperalgesia and upregulated theexpression of Endophilin Ⅰ. The upregulation of Endophilin Ⅰ was in accordance with the development of hyperalgesiaplayed a role in the endocytosis of μopioid receptor,which suggested that the upregulation of Endophilin Ⅰ may be a underlying mechanism of hyperalgesia induced by remifentanil.
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