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Antifungal effect of the active fraction of Dryopteris fragrans on Trichophyton rubrum in vitro
Author(s): 
Pages: 78-82
Year: Issue:  1
Journal: Journal of Guangdong College of Pharmacy

Keyword:  Dryopteris fragransTrichophyton rubrumtest tube dilution methodbroth microdilution methodminimum inhibitory concentrationminimum fungicide concentration;
Abstract: 目的 采用试管稀释法和CLSI M38-A2微量液基稀释法研究香鳞毛蕨有效部位(简称DF)对红色毛癣菌的体外抗真菌作用,并比较两种方法的一致性.方法 利用两种方法测定DF对红色毛癣菌标准株(6株)和临床分离菌株(7株)的最低抑菌浓度(MIC)和最低杀菌浓度(MFC).结果 试管稀释法测定DF对13株红色毛癣菌的MIC范围为生药0.469~ 15.0 mg/mL;MFC范围为生药0.938~ 15.0 mg/mL.其中DF对标准菌株MIC范围为生药0.469 ~ 7.5 mg/mL,几何均值为2.652 mg/mL,MFC范围为生药0.938~ 15.0 mg/mL,几何均值为3.750 mg/mL.微量稀释法测定DF对红色毛癣菌标准菌株的MIC范围为生药0.312 5~5.0 mg/mL,几何均值为1.25 mg/mL;其MFC范围为生药1.25 ~ 20.0 mg/mL,几何均值为5.0 mg/mL.结论 香鳞毛蕨有效部位对红色毛癣菌有抑菌作用和杀菌作用,两种方法的测定结果有较好的一致性.
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