Expression of Zinc Transporter ZIP8 in Osteoarthritis and the Underlying Mechanism
Author(s): SHI Da, ZHAO Cong-zhe, CHAI Hui-bin, LI Zhe, HAN Wei-hua, SUN Yin-di, Department of Chinese Medicine Orthopedics, Xi’an Jiaotong University Medical College Red Cross Hospital
Pages: 2061-2065+2126
Year: 2016 Issue: 11
Journal: Progress in Modern Biomedicine
Keyword: Osteoarthritis; Zinc transporter; Chondrocyte; Matrix metalloproteinases;
Abstract: Objective: To investigate the expression of zinc transporter ZIP8 in osteoarthritis(OA) and its effect on the growth and viability of chondrocyte and the expression of matrix metalloproteinases(MMPs).Methods: The serum and cartilage tissues were collected from 20 osteoarthritis patients(OA group) and 20 non-osteoarthritis patients(control group).Concentrations of zinc-ion in serum and cartilage tissues were measured by atomic absorption spectrophotometer.Cell growth and viability was detected by MTT assay.ZIP8 gene silencing was achieved by small interfering RNA(si RNA).The m RNA expression levels of ZIP8,MMP3,MMP9,MMP12 and MMP13 were measured by quantitative real-time PCR.The protein expression levels of ZIP8,MMP3,MMP9,MMP12 and MMP13 were detected by Western blot analysis.Results: The concentrations of zinc-ion were elevated in the serum and cartilage tissues of OA group as compared with control group(P<0.01).The m RNA(P<0.05) and protein(P<0.01) expression of ZIP8 were both significantly increased in cartilage tissues of OA group compared with control group.ZIP8 si RNA effectively silenced the expression of ZIP8 in chondrocytes(P<0.01).ZIP8 silencing significantly promoted cell growth of chondrocytes isolated from OA patients(P<0.05) and markedly inhibited the expression of MMPs including MMP3,MMP9,MMP12 and MMP13(P<0.05).Conclusions: ZIP8 is closely related to osteoarthritis,and ZIP8 gene expression silencing was capable of increasing cell growth and viability of chondrocytes and decreasing gene expression of MMPs which provides a novel target for treatment of OA in future.
Pages: 2061-2065+2126
Year: 2016 Issue: 11
Journal: Progress in Modern Biomedicine
Keyword: Osteoarthritis; Zinc transporter; Chondrocyte; Matrix metalloproteinases;
Abstract: Objective: To investigate the expression of zinc transporter ZIP8 in osteoarthritis(OA) and its effect on the growth and viability of chondrocyte and the expression of matrix metalloproteinases(MMPs).Methods: The serum and cartilage tissues were collected from 20 osteoarthritis patients(OA group) and 20 non-osteoarthritis patients(control group).Concentrations of zinc-ion in serum and cartilage tissues were measured by atomic absorption spectrophotometer.Cell growth and viability was detected by MTT assay.ZIP8 gene silencing was achieved by small interfering RNA(si RNA).The m RNA expression levels of ZIP8,MMP3,MMP9,MMP12 and MMP13 were measured by quantitative real-time PCR.The protein expression levels of ZIP8,MMP3,MMP9,MMP12 and MMP13 were detected by Western blot analysis.Results: The concentrations of zinc-ion were elevated in the serum and cartilage tissues of OA group as compared with control group(P<0.01).The m RNA(P<0.05) and protein(P<0.01) expression of ZIP8 were both significantly increased in cartilage tissues of OA group compared with control group.ZIP8 si RNA effectively silenced the expression of ZIP8 in chondrocytes(P<0.01).ZIP8 silencing significantly promoted cell growth of chondrocytes isolated from OA patients(P<0.05) and markedly inhibited the expression of MMPs including MMP3,MMP9,MMP12 and MMP13(P<0.05).Conclusions: ZIP8 is closely related to osteoarthritis,and ZIP8 gene expression silencing was capable of increasing cell growth and viability of chondrocytes and decreasing gene expression of MMPs which provides a novel target for treatment of OA in future.
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