Effects of Jiangtang Xiaoke Granules on JNK signal pathway in renal tissues of KKAy mice with type 2 diabetic
Author(s): YU Na, ZUO Jia-cheng, MU Qian-qian, ZHANG Yi, AN Hong, ZHAO Dan-dan, MO Fang-fang, GAO Si-hua, Beijing University of Chinese Medicine
Pages: 1801-1804
Year: 2016 Issue: 5
Journal: China Journal of Traditional Chinese Medicine and Pharmacy
Keyword: Jiangtang Xiaoke Granules; Diabetes nephropathy; JNK signal pathway;
Abstract: Objective: To investigate the effect of Jiangtang Xiaoke Granules(JTXKG) on JNK signal pathway in renal tissues of type 2 diabetic KKAy mice model induced by high fat diet. Methods: 40 KKAy mice were randomly divided into 5 groups based on the glucose level and body weight: the model group, pioglitazone group, JTXKG high, middle and low dose groups(7.0, 3.5, 1.75g/kg), 8 rats in each group, besides, 8 C57BL/6J mice were used as normal group. Rats in the normal group and model group were administrated with distilled water, while the others were gavaged with corresponding drugs. After 10 weeks of intervention, the levels of fasting blood glucose(FBG), urea nitrogen(BUN), creatinine(Scr), albumin(ALB) and total protein(TP) in the serum were detected. The m RNA level and protein level of JNK1, JNK2, P-JNK in renal tissues were detected with RT-PCR and Western Blot respectively. Pathomorphologic changes of each group were demonstrated by HE staining. Results: Compared with rats in the normal group, levels of FBG, BUN and Scr were significantly increased, while the ALB and TP levels were significantly decreased in diabetic model(P<0.05). The m RNA level of JNK and protein levels of JNK1, JNK2 and P-JNK were significantly increased in diabetic model. After 10 weeks of drug intervention, the levels of FBG, BUN and Scr significantly decreased, and the plasma protein level significantly increased excepted the low dose CHD group, compared with the model group(P<0.01, P<0.05). In addition, all these drugs could down-regulate m RNA level of JNK and protein levels of JNK1, JNK2 and P-JNK in renal tissues of diabetic model(P<0.01, P<0.05). Treatment with JTXKG alleviated the pathological changes of renal tissues in HE staining. Conclusion: JTXKG could protect the renal injury of diabetes rats, which may be related to the inhibition of JNK signaling pathway activation.
Pages: 1801-1804
Year: 2016 Issue: 5
Journal: China Journal of Traditional Chinese Medicine and Pharmacy
Keyword: Jiangtang Xiaoke Granules; Diabetes nephropathy; JNK signal pathway;
Abstract: Objective: To investigate the effect of Jiangtang Xiaoke Granules(JTXKG) on JNK signal pathway in renal tissues of type 2 diabetic KKAy mice model induced by high fat diet. Methods: 40 KKAy mice were randomly divided into 5 groups based on the glucose level and body weight: the model group, pioglitazone group, JTXKG high, middle and low dose groups(7.0, 3.5, 1.75g/kg), 8 rats in each group, besides, 8 C57BL/6J mice were used as normal group. Rats in the normal group and model group were administrated with distilled water, while the others were gavaged with corresponding drugs. After 10 weeks of intervention, the levels of fasting blood glucose(FBG), urea nitrogen(BUN), creatinine(Scr), albumin(ALB) and total protein(TP) in the serum were detected. The m RNA level and protein level of JNK1, JNK2, P-JNK in renal tissues were detected with RT-PCR and Western Blot respectively. Pathomorphologic changes of each group were demonstrated by HE staining. Results: Compared with rats in the normal group, levels of FBG, BUN and Scr were significantly increased, while the ALB and TP levels were significantly decreased in diabetic model(P<0.05). The m RNA level of JNK and protein levels of JNK1, JNK2 and P-JNK were significantly increased in diabetic model. After 10 weeks of drug intervention, the levels of FBG, BUN and Scr significantly decreased, and the plasma protein level significantly increased excepted the low dose CHD group, compared with the model group(P<0.01, P<0.05). In addition, all these drugs could down-regulate m RNA level of JNK and protein levels of JNK1, JNK2 and P-JNK in renal tissues of diabetic model(P<0.01, P<0.05). Treatment with JTXKG alleviated the pathological changes of renal tissues in HE staining. Conclusion: JTXKG could protect the renal injury of diabetes rats, which may be related to the inhibition of JNK signaling pathway activation.
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