Author(s): XIAO Jiao, SONG Jun-yu, PAN Ying-ni, LIU Li-li, CHEN Gang, LI Ning, Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang Guancheng Pharmaceutical Co., Ltd.
Pages: 591-594
Year: 2016 Issue:  5
Journal: World Phytomedicines
Keyword:  Stephania japonica(Thunb.) Miers; Stephania epigaea Lo; Stephania cepharantha Hayata; cepharanthine; HPLC;
Abstract: Objective To establish an HPLC method for determination of cepharanthine in the plants of Stephania Lour. from various habitats and parts. Methods The determination was carried out on Angela C18 column(250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water(52∶48, 0.5% triethylamine and 0.01% phosphoric acid). The column temperature was set at 30 ℃ at a flow rate of 1.0 m L/min. The injection volume was 20 μL. Results Cepharanthine had a good linearity in the ranges of 9 — 216 μg/mL(r = 0.999 7). The average recovery rate was 96.0% with RSD value at 2.0%. Contents of cepharanthine in Stephania japonica sample(No.13) from Hunan province and S. cepharantha sample(No.17) from Guangxi province were the highest. Contents of cepharanthine in various plant parts of samples(No.12 — 15) were different, and that in S. japonica from Hunan province were generally found in the stem, which content reached to 0.013 9%. Conclusion The established method is simple, sensitive, and highly reproducible, which is suitable for the determination of cepharantine in S. japonica, S. epigaea, and S. cepharantha.