Author(s): SUN Xing-hua, LI Sheng-hui, DING Qing-long, DI Liu-qing, HUANG Jia-fu, LI Jun-song, ZU Qiang, School of Pharmacy, Nanjing University of Chinese Medicine, Drug Research Department of Army Psychological Disease Prevention and Treatment Center, Chinese PLA 102 Hospital, Jiangsu Engineering Research Center for Efficient Delivery System of TCM, Nanjing Engineering and Technology Research Center of TCM Pellets, Department of Pharmacy, Chinese PLA 102 Hospital
Pages: 2843-2847+2864
Year: 2015 Issue:  24
Journal: Chinese Journal of New Drugs
Keyword:  fluoxetine hydrochloride; ethosome; gel; transdermal penetration;
Abstract: Objective: To prepare fluoxetine hydrochloride ethosome gel and to investigate its transdermal penetration in vitro. Methods: Fluoxetine hydrochloride ethosomes were prepared by an ethanol injection method.The formulation and the preparation method of ethosomes were optimized by orthogonal experiment using entrapment efficiency( EE) and particle size as indexes. The particle size,zeta potential and entrapment efficiency were evaluated,and the carbomer was added as the base for preparation of the ethosome gel. The penetration of fluoxetine hydrochloride ethosome gel through rat skin was evaluated by Franz’s cell. The concentration of fluoxetine hydrochloride was determined by HPLC. The accumulated permeation amounts and steady permeation rate of fluoxetine hydrochloride were calculated. Results: The color of fluoxetine hydrochloride ethosome was light blue,the average diameter was( 77. 3 ± 1. 49) nm,and the EE was( 60. 4 ± 1. 76) %. The permeation rate of fluoxetine hydrochloride ethosome gel [( 5. 13 ± 0. 78) μg·cm- 2·h- 1]was 1. 31 and 4. 31 times higher than those of general gel and control gel,respectively. Conclusion: The preparation technique of fluoxetine hydrochloride ethosome gel is feasible with stable and controllable quality,and can improve transdermal penetration.