Relationship between increased nuocytes and GITR-GITRL expression levels in lung tissue of asthmatic mice
Author(s): ZHANG Meng-ying, XU Yun-yun, WU Jing, ZHANG Pan, ZHANG Dan-yi, PENG Jing-jing, QI Chen, JI Xiao-yun, XIA Sheng, SU Zhao-liang, WANG Sheng-jun, XU Hua-xi, Department of Immunology, School of Medicine, Jiangsu University
Pages: 277-281
Year: 2015 Issue: 4
Journal: Journal of Zhenjiang Medical College
Keyword: nuocytes; GITR; GITRL; asthma; mice;
Abstract: Objective: To detect the changes of numbers of nuocytes and levels of GITR-GITRL in the lung of asthmatic mice,analyze the relationship between them,then to explore the changes of Th2-polarized micro-environment at the locus of allergic inflammation when with asthma. Methods: Eight mice were devided into 2 groups randomly,asthma models were sensitized with OVA on 1st,11 st day,then nebulized inhalation with OVA on day 22-26,control group with PBS. Flow cytometry was used to detect numbers of nuocytes and cells which express GITR; qRT-PCR was also used to test the mRNA levels of GITR-GITRL,molecules and cytokines associated with nuocytes; pearson correlation analysis was used to detect the correlation between GITR-GITRL and nuocytes. Results: Compared with the control group,model group showed high levels of nuocytes and GITR-GITRL,meanwhile,with increased mRNA levels of GITRL,Foxp3,RORα,ICOS,ST2,IL-5 and IL-13; and there was a positive correlation between GITR expression and RORα,ST2,IL-5,IL-13 expression. Conclusion: Nuocytes and the expression of GITR-GITRL increased synchronously in lung of asthmatic mice,which suggested that the up-regulation of T cell-mediated immune response by GITR-GITRL may be associated with Th2-biased response and polarization of nuocytes.
Pages: 277-281
Year: 2015 Issue: 4
Journal: Journal of Zhenjiang Medical College
Keyword: nuocytes; GITR; GITRL; asthma; mice;
Abstract: Objective: To detect the changes of numbers of nuocytes and levels of GITR-GITRL in the lung of asthmatic mice,analyze the relationship between them,then to explore the changes of Th2-polarized micro-environment at the locus of allergic inflammation when with asthma. Methods: Eight mice were devided into 2 groups randomly,asthma models were sensitized with OVA on 1st,11 st day,then nebulized inhalation with OVA on day 22-26,control group with PBS. Flow cytometry was used to detect numbers of nuocytes and cells which express GITR; qRT-PCR was also used to test the mRNA levels of GITR-GITRL,molecules and cytokines associated with nuocytes; pearson correlation analysis was used to detect the correlation between GITR-GITRL and nuocytes. Results: Compared with the control group,model group showed high levels of nuocytes and GITR-GITRL,meanwhile,with increased mRNA levels of GITRL,Foxp3,RORα,ICOS,ST2,IL-5 and IL-13; and there was a positive correlation between GITR expression and RORα,ST2,IL-5,IL-13 expression. Conclusion: Nuocytes and the expression of GITR-GITRL increased synchronously in lung of asthmatic mice,which suggested that the up-regulation of T cell-mediated immune response by GITR-GITRL may be associated with Th2-biased response and polarization of nuocytes.
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