Author(s): ZOU Chun-cai, YAN Hai-yan, Department of Pharmacy, Wannan Medical College
Pages: 341-345+350
Year: 2016 Issue:  2
Journal: International Journal of Pharmaceutical Research
Keyword:  evolving window orthogonal projection analytical method; Gualou Xiebai Baijiu Decoction; medicinal ingredi ents in prescription; peaks matching;
Abstract: Objective To explore whether evolving window orthogonal projection analytical method can be used in the chromatographic peaks matching between traditional Chinese medicine preparation and its medicinal ingredients in prescription. Methods The fingerprints of Gualou Xiebai Baijiu Decoction and the herbs(Gualou and Xiebai)in its prescription were established by using high performance liquid chromatography-photodiode array detector(HPLC-PDAD), and the HPLC peaks in fingerprints of Gualou Xiebai Baijiu Decoction and the herbs in its prescription were matched according to the principle of evolving window orthogonal projection analytical method. Results Evolving window orthogonal projection analytical method could match the chromatogram peaks well between different fingerprints of Gualou Xiebai Baijiu Decoction and the herbs in its prescription. There were 20 common peaks between the ethyl acetate extraction of Gualou Xiebai Baijiu Decoction and Gualou, and 9 common peaks between the n-butanol extraction of Gualou Xiebai Baijiu Decoction and Xiebai. Conclusion Evolving window orthogonal projection analytical method can be used in the chromatographic peaks matching between traditional Chinese medicine preparation and its medicinal ingredients in prescription.