Determination of Iodate in Salt Using HPLC with Post-column Derivation
Author(s): ZHANG Zi-lu, ZHOU Guo-qing, MA Yue, HUANG Ping
Pages: 136-139
Year: 2016 Issue: 1
Journal: China Condiment
Keyword: salt; post-column derivation; I-3;
Abstract: A method is developed to determine iodate in salt using HPLC-UV with post-column derivation based on I-3 with high molar absorption coefficient produced via the reaction of IO-3 and I-in acid.A Hamilton PRP-X100 anion exchange resin column (Φ4.6 ×250 mm)is used as analytical column and 1 mol/L NaCl as mobile phase.The mixture solution of KI and HCl is used as post-column derivation reagent.When the flow rate of mobile phase and post-column derivation reagent is 1 mL/L,the retention time of IO-3 is 3.5 min.The standard curve for the method with the injection volume of 50 μL is obtained and the regression equation is A=6.36×10 -2 +0.469×C (μg/g for I)with r2 = 0.99977.Comparatively,for the method to monitor the absorbance of iodate at 220 nm directly,the method with post-column derivation has a higher sensitivity.The method is validated by analysis of iodate in standard salt and the bias between content determined and standard value is less than 1.7%.A kind of commercial salt samples is analyzed and the content of iodate is according with the declared value.The minimum detectable limit for the method is 0.18 μg/g.
Pages: 136-139
Year: 2016 Issue: 1
Journal: China Condiment
Keyword: salt; post-column derivation; I-3;
Abstract: A method is developed to determine iodate in salt using HPLC-UV with post-column derivation based on I-3 with high molar absorption coefficient produced via the reaction of IO-3 and I-in acid.A Hamilton PRP-X100 anion exchange resin column (Φ4.6 ×250 mm)is used as analytical column and 1 mol/L NaCl as mobile phase.The mixture solution of KI and HCl is used as post-column derivation reagent.When the flow rate of mobile phase and post-column derivation reagent is 1 mL/L,the retention time of IO-3 is 3.5 min.The standard curve for the method with the injection volume of 50 μL is obtained and the regression equation is A=6.36×10 -2 +0.469×C (μg/g for I)with r2 = 0.99977.Comparatively,for the method to monitor the absorbance of iodate at 220 nm directly,the method with post-column derivation has a higher sensitivity.The method is validated by analysis of iodate in standard salt and the bias between content determined and standard value is less than 1.7%.A kind of commercial salt samples is analyzed and the content of iodate is according with the declared value.The minimum detectable limit for the method is 0.18 μg/g.
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