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gao xiao ye xiang fa ce ding tu mei su zhu she ye de han liang
Pages: 12-13
Year: Issue:  3
Journal: Henan Journal of Animal Husbandry and Veterinary Medicine

Keyword:  土霉素高效液相含量测定;
Abstract: 针对土霉素注射液现行标准中含量测定方法检测周期较长的缺点,建立一种高效液相色谱测定方法方法.色谱条件:Waters XBridgeTMC18色谱柱,流动相为醋酸铵溶液[0.25 mol/L醋酸铵溶液-0.05 mol/L乙二胺四醋酸二钠-三乙胺(100∶10∶1)用醋酸调节pH值至7.5]-乙腈(88∶12),流速为1.0 ml/min,柱温为30℃,检测波长为280 nm.土霉素浓度在10~500μg/ml的范围内,峰面积与浓度呈良好的线性关系,R2=1.该方法简便、准确、快速,可用于土霉素注射液半成品含量的测定.
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