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Effects of Exogenous Spd on the Pollen Tube Growth and Stylar Protein Expression During Pollination and Fertilization of Yali Pear
Pages: 22-28
Year: Issue:  1
Journal: Acta Agriculturae Boreali-Sinica

Keyword:  SpermidineYali pearPollen tubeStylar protein;
Abstract: Yali pear belongs to the kind of gametophytic self-incompatibility,the percentage of self-pollinated fruit set is extremely low.Our previous research showed that sprayed polyamine in a concentration range at flores-cence could break self-incompatibility of Yali pear.In order to explore the physiological and biochemical mecha-nism,the effects of exogenous Spd on the pollen tube growth and stylar protein expression of the Yali pear were stud-ied by two-dimensional gel electrophoresis and mass spectrum analysis technology.The main results were as follows:The pollen tube length were extremely significantly increased after suitable concentration of Spd treatment,the best effects of exogenous Spd were 0.25 mmol/L Spd treatment,the number of pollen tube reached to the bottom of style were the most after 72 hours of treatment.Three specific proteins were found in the electrophoretogram.Two protein spots were obtained by the mass spectrometry results,which were predicted protein and putative protein respectively.
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