Optimization of Enzymatic Extraction and Nitrite Scavenging Capacity of Flavonoids from Peony Leaves
Author(s): LI Peiyan, HAN Sihai, LUO Denglin, ZHAO Shengjuan, LIU Jianxue, YIN Fei, College of Food Science and Biotechnology, Henan University of Science and Technology, College of Agronomy, Henan University of Science and Technology
Pages: 77-81
Year: 2016 Issue: 6
Journal: Food Science
Keyword: peony leaves; flavanoids; cellulase; nitrite; scavenging;
Abstract: Cellulose-assisted extraction was applied to the extraction of flavanoids from peony leaves. Effects of enzyme dosage, p H, temperature and hydrolysis time on the extraction efficiency of flavanoids were investigated. The optimal hydrolysis conditions obtained by orthogonal array experiments for the extraction of flavonoids were as follows: enzyme dosage, 12.5 U/m L; initial p H, 4.5; temperature, 45 ℃; and hydrolysis time, 4 h. Under these conditions, the extraction yield of flavanoids was 2.43%. Flavanoids from peony leaves were able to scavenge nitrite. The optimal reaction conditions for nitrite scavenging by the flavanoids were found to be 20 min reaction at 70 ℃ and p H 4.0 after addition of 25 m L(10 μg sodium nitrate) of the radical scavenger, resulting in a scavenging rate of 62.15%.
Pages: 77-81
Year: 2016 Issue: 6
Journal: Food Science
Keyword: peony leaves; flavanoids; cellulase; nitrite; scavenging;
Abstract: Cellulose-assisted extraction was applied to the extraction of flavanoids from peony leaves. Effects of enzyme dosage, p H, temperature and hydrolysis time on the extraction efficiency of flavanoids were investigated. The optimal hydrolysis conditions obtained by orthogonal array experiments for the extraction of flavonoids were as follows: enzyme dosage, 12.5 U/m L; initial p H, 4.5; temperature, 45 ℃; and hydrolysis time, 4 h. Under these conditions, the extraction yield of flavanoids was 2.43%. Flavanoids from peony leaves were able to scavenge nitrite. The optimal reaction conditions for nitrite scavenging by the flavanoids were found to be 20 min reaction at 70 ℃ and p H 4.0 after addition of 25 m L(10 μg sodium nitrate) of the radical scavenger, resulting in a scavenging rate of 62.15%.
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