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The Isolation and Identification of Bovine Viral Diarrhea Virus-Mucosal Disease Virus in Xinjiang
Author(s): 
Pages: 2335-2343
Year: Issue:  12
Journal: Xinjiang Agricultural Sciences

Keyword:  bovinebovine viral diarrhea virus-mucosal disease virusisolationidentification;
Abstract: [目的]了解新疆地区BVDV毒株的生物型和基因型.[方法]采集新疆石河子和伊犁地区疑似感染病毒性腹泻-黏膜病病毒牛的新鲜粪便174份,应用RT-PCR方法对其进行检测,将检测结果为阳性的样品接种于MDBK细胞上培养,电镜观察,测定毒价,使用RT-PCR方法对细胞培养物进行鉴定,并提取质粒进行测序,对结果分析.[结果]该毒株可使MDBK细胞发生病变,扩增出一条286 bp目的片段,经同源性和遗传进化分析,确定为BVDV-1型毒株.[结论]分离到一株BVDV-1型新疆毒株,可使MDBK细胞发生病变,病毒粒子直径约为20~ 40 nm,毒株TCID50 10-4.5/0.1 mL.
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