Effect of Total Saponins of Laser Knife Combined with Acupoint Injection of Radix Clematidis on RF and PGE2 of Model Rats with Knee Osteoarthritis
Author(s): YAN Mei, GUO Li-hong
Pages: 71-74
Year: 2015 Issue: 12
Journal: Journal of Clinical Acupuncture and Moxibustion
Keyword: Laser needle knife; Total saponins of Radix Clematidis; Knee osteoarthritis; PGE2; Rheumatoid factors; Acupoint injection;
Abstract: Objective:Establishing the experimental operation for model rats with knee osteoarthritis, to observe the effect of laser needle knife combined with acupoint injection of Radix Clematidis saponins on serum rheuma-toid factor( RF) RA and PGE2 , and to investigate its mechanism of action.Methods:60 rats were randomly di-vided into a blank control group,a model group,a laser acupuncture group,an acupoint injection group and a combined treatment group, except the blank control group in other four groups animals received the left knee joint cavity injection of Freundˊs complete adjuvant (0.1 ml).After the success of modeling,the model group was injected with saline;laser needle knife group received electroacupuncture combined with laser needle knife treatment, electroacupuncturing at Guanyuan, Zusanli, Pishu, Qihai, Yanglingquan, Shenshu, Ganshu for 20 min;the acupoint injection group received the left knee joint cavity acupoint injection(50mg/kg);comprehen-sive treatment method was used for acupoint injection group and combined treatment group; each group was treated for 14 days.Determine animal articular effusion of hyaluronic acid ( HA) and interleukin 6 IL-and prostaglandin E2(PGE2), serum rheumatoid factor (rheumatoid, factor, RF) IgM-RF, IgA-RF positive rate, the positive expression rate of MMP-1 cells in pathological articular cartilage.Results:The laser knife combined with acupoint injection of Radix Clematidis saponins could significantly decrease the IL-6, PGE2 , IgM-RF, IgA-RF and could MMP-1 and increase HA.Compared with the model group, the laser needle knife group and acupoint injection group had a significant difference(P<0.05).Conclusion:Laser knife com-bined with acupoint injection of Radix Clematidis saponins can be effective in the treatment of rats with knee os-teoarthritis,and its mechanism may be related to the laser acupotomy knee joint adhesion in inflammation tissue, electroacupuncture stimulation,reduction of IL-6 and PGE2 and other inflammatory factors stimulation, increase of HA content in articular cavity.Prevent rheumatoid factor from binding with antigen IgM and IgA, and de-crease the combination of IgM-RF and IgA-RF,reduce the occurrence of allergic reactions and play a syner-gistic effect.
Pages: 71-74
Year: 2015 Issue: 12
Journal: Journal of Clinical Acupuncture and Moxibustion
Keyword: Laser needle knife; Total saponins of Radix Clematidis; Knee osteoarthritis; PGE2; Rheumatoid factors; Acupoint injection;
Abstract: Objective:Establishing the experimental operation for model rats with knee osteoarthritis, to observe the effect of laser needle knife combined with acupoint injection of Radix Clematidis saponins on serum rheuma-toid factor( RF) RA and PGE2 , and to investigate its mechanism of action.Methods:60 rats were randomly di-vided into a blank control group,a model group,a laser acupuncture group,an acupoint injection group and a combined treatment group, except the blank control group in other four groups animals received the left knee joint cavity injection of Freundˊs complete adjuvant (0.1 ml).After the success of modeling,the model group was injected with saline;laser needle knife group received electroacupuncture combined with laser needle knife treatment, electroacupuncturing at Guanyuan, Zusanli, Pishu, Qihai, Yanglingquan, Shenshu, Ganshu for 20 min;the acupoint injection group received the left knee joint cavity acupoint injection(50mg/kg);comprehen-sive treatment method was used for acupoint injection group and combined treatment group; each group was treated for 14 days.Determine animal articular effusion of hyaluronic acid ( HA) and interleukin 6 IL-and prostaglandin E2(PGE2), serum rheumatoid factor (rheumatoid, factor, RF) IgM-RF, IgA-RF positive rate, the positive expression rate of MMP-1 cells in pathological articular cartilage.Results:The laser knife combined with acupoint injection of Radix Clematidis saponins could significantly decrease the IL-6, PGE2 , IgM-RF, IgA-RF and could MMP-1 and increase HA.Compared with the model group, the laser needle knife group and acupoint injection group had a significant difference(P<0.05).Conclusion:Laser knife com-bined with acupoint injection of Radix Clematidis saponins can be effective in the treatment of rats with knee os-teoarthritis,and its mechanism may be related to the laser acupotomy knee joint adhesion in inflammation tissue, electroacupuncture stimulation,reduction of IL-6 and PGE2 and other inflammatory factors stimulation, increase of HA content in articular cavity.Prevent rheumatoid factor from binding with antigen IgM and IgA, and de-crease the combination of IgM-RF and IgA-RF,reduce the occurrence of allergic reactions and play a syner-gistic effect.
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