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RESEARCH OF CHARACTERISTICS AND FUNCTIONS OF ENRICHMENT OF Pb2+ BY RECOMBINANT FERRITIN FROM PLASCOLOSOMA ESCULENTA
Author(s): 
Pages: 1358-1364
Year: Issue:  6
Journal: Oceanologia et Limnologia Sinica

Keyword:  Phascolosoma esculentarecombinant ferritinenrichment lead ionultra-structuremouse osteoblast precursor cells;
Abstract: The recombinant ferritin from Phascolosoma esculenta were exposed to the same concentration of different heavy metals to absorb them. The 8 kinds of heavy metals were Pb2+, Fe3+, Cd2+, Cr3+, Cu2+, Mn2+, Sn2+ and Zn2+. Transmission electron microscopy(TEM) and Fourier transform infrared spectroscopy(FTIR) were applied to study the ultra-structure and the change of groups structure. It compared to natural horse spleen ferritin with the same processing. The results indicated that the ultra-structure of ferritin was altered after trapping different kinds of heavy metals. Recombinant ferritin groups changed after trapping different heavy metals. There were some specific groups for binding heavy metals, including-CH3,-CH2-,-CH-,-NH-,-SO3-,-COOH,-S=S-,-C-S-C etc., besides some non-specific amides characteristic peaks and ferritin characteristic peaks. Further experiments verified that ferritin could protect the mouse osteoblast precursor cells MRC3T3-E1 through enrichment of Pb2+ and other heavy metal ions, and the cell mortality was significantly lower(P<0.05).
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