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CHARACTERIZATION AND EXPRESSION ANALYSIS OF COMPLEMENT REGULATOR FACTOR CFH AND CFHR2 IN LARGE YELLOW CROAKER LARIMICHTHYS CROCEA
Author(s): 
Pages: 1405-1412
Year: Issue:  6
Journal: Oceanologia et Limnologia Sinica

Keyword:  Large yellow croaker Larimichthys croceacomplement regulator factor CFHcomplement regulator factor CFHR2molecular characterizationexpression analysis;
Abstract: The complement system is an important component in fish innate immunity which can recognize, facilitate the elimination of pathogens, promote the function of phagocyte, activate immune cell as well as modulate the adaptive immune response. In the immune tissues, about half of the complement components exist as regulating factors, which play key role in complement activation. CFH and CFHR2 are important regulator factors in alternative pathways of complement system and maintain component system function well. Although the complement system of Larimichthys crocea has been extensively studied, the expression and evolution of CFH and CFHR2 remain unknown. In this study, we reported the primary sequence of Larimichthys crocea complement regulator factor H and HR2. CFH open reading frame(ORF) is 1332 bp and encodes for 443 amino acids with a putative signal peptide of 24 amino acid residues. CFHR2 open reading frame(ORF) is 1170 bp and encodes for 339 aa with a putative signal peptide of 32 amino acid residues. Deduced amino acid sequence of the structure analysis indicated that the L. crocea CFH and CFHR2 have typical characteristics of RCA protein family, possessing several conservative CCP structures(complement control protein). Real-time PCR analysis showed that the Liver displayed the most abundant expression level of three genes among the tested tissues of the healthy individuals. We found that Vibrio alginolyticus exposure up-regulated the expressions significantly in the early induction. As the change of infection time, the expression present different rising trend. The result showed that CFH and CFHR2 gene are very sensitive to pathogenic substances, which indicated that complement regulator factors may play an significant role in immunologic mechanisms of croaker.
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