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IDENTIFICATION AND ISOLATION OF A NOVEL BACTERIAL PATHOGEN(ENTEROBACTER CLOACAE) FROM DISEASED LARVAE OF GIANT FRESHWATER PRAWN MACROBRACHIUM ROSENBERGII
Author(s): 
Pages: 1467-1477
Year: Issue:  6
Journal: Oceanologia et Limnologia Sinica

Keyword:  Macrobrachium rosenbergii larvaenovel pathogenEnterobacter cloacaeisolation and identificationantibiotic sensitivity test;
Abstract: In 2010, a new disease broke out in most Macrobrachium rosenbergii hatcheries in Huzhou, Zhejiang Province. The cumulative mortality of larvae was 70%—80%, resulting in great economic losses. Six dominant bacterium(NTH01, NTH02, NTH03, 316 D, 316 X, and 316C) were isolated from the homogenate of infected larvae. The morphology of isolated bacterium from Nutrient Agar was consistent. An artificial infection experiment was performed to test the pathogenicity of representative strain NTH01. The result shows that healthy larvae could be infected by strain NTH01. In morphological, physiological, and biochemical aspects, strain NTH01 was identified as Enterobacter cloacae. A PCR method established for E. cloacae detection was used to detect the other 5 dominant bacteria, showing a positive result. In 2011, samples of larvae and adult shrimp from 4 infected hatcheries were detected by the PCR method, the PCR showed a positive result in diseased larvae samples, but negative in healthy larvae. Although positive detection results were also found in hepatopancreas and gut, adult shrimp did not showed a diseased symptoms. Strain NTH01 was resistant to 30 kinds of commonly antibiotics, such as cefoxitin, enrofloxacin, amikacin, and so on. The results of drug sensitivity test show that enrofloxacin, ofloxacin, gentamicin, neomycin, doxycycline and aztreonam were most effective against the pathogenic bacterium. However, the treatment of E. cloacae infections is difficult and expensive. Therefore, a specific pathogen-free environment for larvae culture shall be constructed to prevent and control the disease caused by E. cloacae.
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