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RAPID ENRICHMENT OF FOUR COMMON PATHOGENIC VIBRIO IN THE OCEAN WITH IMMUNOMAGNETIC NANOBEADS
Author(s): 
Pages: 1478-1486
Year: Issue:  6
Journal: Oceanologia et Limnologia Sinica

Keyword:  immunomagnetic nanobeadspathogenic vibrioenrichmentcapture rate;
Abstract: To develop a quick separation and enrichment of pathogenic vibrio in the ocean with immunomagnetic nanobeads(IMBs), for which four vibrios(Vibrio parahaemolyticus, Vibrio harveyi, Vibrio vulnificus, and Vibrio splendidus)were tested. We evaluated the sensitivity, specificity, and anti-interference ability of each IMB made from these vibrios. Results show that for the four types of IMBs, the optimal amount of antibody conjugate was 300, 600, 150, and 150μg, respectively. The optimum capturing conditions were, in the turns of IMS amount(in mg) / immunoreaction(in min) / separation(in min) / p H: 3/45/3/7.4 for V. parahaemolyticus, 3/45/1/6.0 for V. harveyi, 12/60/5/8.0 for V. vulnificus, and 6/30/3/7.4 for V. splendidus. In pure culture, the capture limit could reach 100 CFU/m L for all four IMBs. In our test on nine bacteria, the capture rate on target bacteria could reach 87% and that on non-target bacteria was below 32%. In addition, the capture rate by IMB of the four vibrios mixed was not affected by other bacteria. Therefore, the IMBs have good sensitivity, specificity, and anti-interference ability, and can reduce detection time considerably, showing good application prospects.
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