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GENETIC VARIABILITY OF mt DNA COI GENES IN SPINIBARBUS HOLLANDI
Author(s): 
Pages: 1509-1515
Year: Issue:  6
Journal: Oceanologia et Limnologia Sinica

Keyword:  Spinibarbus hollandihybridsCOIgenetic variability;
Abstract: To identify phylogenetic variability in Spinibarbus hollandi hybrids from two populations, their mitochondrial cytochrome oxidase subunit I(COI) gene fragments were amplified in PCR(Polymerase Chain Reaction), of which a 1551-bp base-pair nucleotide sequence was examined. Eighteen samples were divided into 6 groups, from which 8 haplotypes and 35 nucleotide polymorphic loci COI gene fragments were recognized and they shared high similarities from 98.1% to 99.9% in nucleotide sequence revealed by sequence divergence and genetic distance analyses, indicating no significant genetic difference among these groups. In five COI haplotypes of the first-generation hybrids, the similarity was 99.4%–99.8% to the female parents and 98.1%–98.5% to the male ones. Therefore, the hybrids inherited more from their maternal parent in mitochondrial COI genes, following strictly the rules of maternal inheritance.
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