Effect of acceleration on oxidative stress of renal calcium oxalate rat models
Author(s): CHEN Kai-kai, GUO He-qing, MU Da-wei, LI Jian-ye, YAN Jing-min, YIN Xing-lin, ZHANG Li-ming, Department of Urology, Air Force PLA General Hospital
Pages: 10-12+31
Year: 2016 Issue: 1
Journal: Journal of Southeast China National Defence Medical Science
Keyword: kidney calculi; calciumoxalate; acceleration; oxidative stress; rats;
Abstract: Objective To investigate the effect of accelerationon on oxidative stress of renal calcium oxalate( Ca Ox) rat models. Methods The models of renal Ca Ox were established with Ethylene Glycol( EG) in drinking water and Ammonium Chloride( AC)by gavage. A total of 40 adult healthy male Wistar rats were randomly and averagely divided into 4 groups( 10 of each) : blank control group( A),stone forming group( B),stone forming with +6G exposure group( C) and +6G exposure naive group( D). Group A was established with drinking water and physiological saline by gavage( 2 m L per day). Group B was established with 1% ethylene glycol in drinking water and 2% ammonium chloride by garage( 2 m L per day). Group C,with the foundation of group B,was exposed in+6G.Group D was exposed in +6G as same as group C. After feeding for 28 days,blood and renal samples were collected. The presence of Ca Ox crystals was scored in left renals under hematoxylin and eosin staining,and the level of malonddialdehyde( MDA),superoxide dismutase( SOD),catalase( CAT),glutathione( GSH) and glutathione Peroxidase( GSH) in the blood and right renal homogenate were measured as indicators of oxidative stress. Results For the formation of Ca Ox crystals,the score of group B and group C were significantly higher than group A and group D( P<0.01),and the score of group C were significantly higher than group B( P< 0. 01).For the parameter of oxidative stress,compared with group A,the concentration of MDA in blood and kidney tissues of group B significantly increased( P<0.01),which significantly increased( P<0.01) after the intervention of +6G( group C). Compared with group A,the content of SOD,CAT and GSH-Px in blood and kidney tissues of group B significantly decreased( P< 0.01),which significantly increased( P<0.01) after the intervention of +6G( group C). Conclusion Acceleration would be one of the risk factor contributed to kidney stone formation. The mechanism is connected with the oxidative damage of renal tissue.
Pages: 10-12+31
Year: 2016 Issue: 1
Journal: Journal of Southeast China National Defence Medical Science
Keyword: kidney calculi; calciumoxalate; acceleration; oxidative stress; rats;
Abstract: Objective To investigate the effect of accelerationon on oxidative stress of renal calcium oxalate( Ca Ox) rat models. Methods The models of renal Ca Ox were established with Ethylene Glycol( EG) in drinking water and Ammonium Chloride( AC)by gavage. A total of 40 adult healthy male Wistar rats were randomly and averagely divided into 4 groups( 10 of each) : blank control group( A),stone forming group( B),stone forming with +6G exposure group( C) and +6G exposure naive group( D). Group A was established with drinking water and physiological saline by gavage( 2 m L per day). Group B was established with 1% ethylene glycol in drinking water and 2% ammonium chloride by garage( 2 m L per day). Group C,with the foundation of group B,was exposed in+6G.Group D was exposed in +6G as same as group C. After feeding for 28 days,blood and renal samples were collected. The presence of Ca Ox crystals was scored in left renals under hematoxylin and eosin staining,and the level of malonddialdehyde( MDA),superoxide dismutase( SOD),catalase( CAT),glutathione( GSH) and glutathione Peroxidase( GSH) in the blood and right renal homogenate were measured as indicators of oxidative stress. Results For the formation of Ca Ox crystals,the score of group B and group C were significantly higher than group A and group D( P<0.01),and the score of group C were significantly higher than group B( P< 0. 01).For the parameter of oxidative stress,compared with group A,the concentration of MDA in blood and kidney tissues of group B significantly increased( P<0.01),which significantly increased( P<0.01) after the intervention of +6G( group C). Compared with group A,the content of SOD,CAT and GSH-Px in blood and kidney tissues of group B significantly decreased( P< 0.01),which significantly increased( P<0.01) after the intervention of +6G( group C). Conclusion Acceleration would be one of the risk factor contributed to kidney stone formation. The mechanism is connected with the oxidative damage of renal tissue.
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