Author(s): Yu-zhen Wang, Wai-ou Zhao, Ya-peng Li, Jing-yuan Wang, Alan G, Mac Diarmid Institute, College of Chemistry, Jilin University, The first hospital of Jilin University
Pages: 211-218
Year: 2016 Issue:  2
Journal: Acta Polymerica Sinica
Keyword:  Folic acid; Quantum dots; DOX; Theranostics; pH-sensitive;
Abstract: By amidation reaction,folic acid( FA) was grafted to hydrophilic polymer poly( glycidyl methacrylate)-g-poly( ethylene glycol))( PGMA-g-PEG). Nuclear magnetic resonance( NMR) and infrared spectroscopy( FTIR) tests showed that polymer PGMA-g-( PEG)( FA) was successfully synthesized and the grafting level of FA was 1. 7%. In order to prepare water-soluble quantum dots,the polymer PGMA-g-( PEG)( FA) was used to modify quantum dots( QDs) by ligand exchange. And using glutaraldehyde,the anti-cancer drug doxorubicin( DOX) was attached to the surface of the water-soluble quantum dots by the imine groups to form the final folic acid targeted theranostics system QDs@ PGMA-g-( PEG)( FA)( DOX). UV-Visible spectra( UV-Vis) showed that the folic acid targeted theranostics system had the characteristic absorption peaks of QDs,DOX,and FA. The result illustrated that the folic acid targeted theranostics system was successfully prepared. The drug release study showed that the system was p H sensitive,had large drug release amount at p H5. 0,and was stable at p H = 7. 4. Hela cells experimental studies showed that compared with the theranostics system,the folic acid targeted theranostics system had greater cytotoxicity and higher concentrations of QDs in the cytoplasm and higher concentrations of DOX in the nucleus. All above,compared with the theranostics system,folic acid targeted theranostics system can be better applied to cell imaging and tumor treatment in the future.