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Comparative Study on Microstructural Changes of Dorsal Root Gsnglia in Female and Male Simulated Weightlessness Rats
Pages: 9-13
Year: Issue:  1
Journal: Space Medicine & Medical Engineering

Keyword:  simulated weightlessnessdorsal root ganglionNissl bodymyelinmyelin basic protein;
Abstract: Objective To study the microstructural changes of dorsal root ganglia( DRG) in female and male simulated weightlessness rats. Methods Twenty male and twenty female 3- month old SD rats were selected.According to their gender,they were randomly divided into 4 groups,2 experimental groups( simulated weightlessness with hindlimbs unloaded) and 2 control groups. After 4 weeks,both side L5-DRG were taken out. Cell morphology with HE staining,Nissl body shape with toluidine blue staining,DRG lamellar structure of myelin with electron microscopy and immune-histo-chemical for MBP content were observed. Results The gaps between DRG cells were widened slightly,ganglion cells and satellite cells were separated partly in hindlimb unloaded groups. Nissl body structure became fluffy,swelling,dyeing uneven,and the structural variants were larger in hindlimb unloaded group than control. Lamellar structure of myelin showed disorder and degenerative changes significantly with electron microscope in hindlimb unloaed groups. Immuno-histo-chemistry showed that in the unloaded groups,the integral optical density( IOD) value of MBP decreased( P < 0.01) significantly compared with the control groups. These differences between female and male hindlimb unloaded groups were more significant( P < 0. 05). Conclusions Simulated weightlessness for 4 weeks can induce serious changes in DRG cells,organelles and myelin basic protein,dorsal root ganglion injury in the female group is more serious than that in the male group. For such differences,aerospace and medical treatment should be individualized.
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