Effect of Zishen Qinggan Formula on Improving Insulin Resistance in GK Rats
Author(s): TIAN Chun-yu, BO Hai-mei, ZHU Liang, LA Xiao-jin, CAO Hui-juan, YAN Xin, YANG Yu-yang, ZHOU Xue-mei, LIU Chang-xiao, LI Ji-an, Tianjin University of Traditional Chinese Medicine, North China University of Science and Technology
Pages: 148-151
Year: 2016 Issue: 2
Journal: Chinese Journal of Experimental Traditional Medical Formulae
Keyword: Zishen Qinggan formula; type 2 diabetes mellitus; insulin resistance;
Abstract: Objective: To study effects of Zishen Qinggan formulaon on blood glucose,glycosylated hemoglobin Alc（Hb Alc）,C-peptide（C-P）,glucagon-like peptide（GLP-1） and α- glucosidase activity in GotoKakizaki（GK） rats. Method: Rats with fasting blood glucose（FBG） > 11. 1 mmol·L-1 were selected and randomly divided into the following groups: diabetic model group,Zishen Qinggan formula low dose group(ig,300mg·kg-1·d-1),middle dose group(ig,600 mg·kg-1·d-1),and high dose group(ig,1 200 mg·kg-1·d-1),and metformin group(ig,85 mg·kg-1·d-1). After 28 d of continuous medication,fasting blood glucose（FBG） was detected and oral glucose tolerance test（OGTT） was done,then Hb Alc,C-P,GLP-1 levels and α- glucosidase activity were detected. Result: Compared with the normal group,FBG and Hb Alc levels in model group were significantly higher（P < 0. 01）. Compared with the model group,FBG,Hb Alc levels and the blood glucose level after 120 min of glucose tolerance were significantly reduced in Zishen Qinggan formula groups（P < 0. 05,P <0. 01）,levels of C-P,and GLP-1 were significantly increased（P < 0. 05,P < 0. 01）,insulin resistance index was reduced（P < 0. 05）, and α-glucosidase activity was inhibited. Conclusion: Zishen Qinggan formula can effectively reduce blood glucose and improve insulin resistance in GK rats,and the action mechanism may be associated with increasing GLP-1 content and inhibiting α- glucosidase activity.
Pages: 148-151
Year: 2016 Issue: 2
Journal: Chinese Journal of Experimental Traditional Medical Formulae
Keyword: Zishen Qinggan formula; type 2 diabetes mellitus; insulin resistance;
Abstract: Objective: To study effects of Zishen Qinggan formulaon on blood glucose,glycosylated hemoglobin Alc（Hb Alc）,C-peptide（C-P）,glucagon-like peptide（GLP-1） and α- glucosidase activity in GotoKakizaki（GK） rats. Method: Rats with fasting blood glucose（FBG） > 11. 1 mmol·L-1 were selected and randomly divided into the following groups: diabetic model group,Zishen Qinggan formula low dose group(ig,300mg·kg-1·d-1),middle dose group(ig,600 mg·kg-1·d-1),and high dose group(ig,1 200 mg·kg-1·d-1),and metformin group(ig,85 mg·kg-1·d-1). After 28 d of continuous medication,fasting blood glucose（FBG） was detected and oral glucose tolerance test（OGTT） was done,then Hb Alc,C-P,GLP-1 levels and α- glucosidase activity were detected. Result: Compared with the normal group,FBG and Hb Alc levels in model group were significantly higher（P < 0. 01）. Compared with the model group,FBG,Hb Alc levels and the blood glucose level after 120 min of glucose tolerance were significantly reduced in Zishen Qinggan formula groups（P < 0. 05,P <0. 01）,levels of C-P,and GLP-1 were significantly increased（P < 0. 05,P < 0. 01）,insulin resistance index was reduced（P < 0. 05）, and α-glucosidase activity was inhibited. Conclusion: Zishen Qinggan formula can effectively reduce blood glucose and improve insulin resistance in GK rats,and the action mechanism may be associated with increasing GLP-1 content and inhibiting α- glucosidase activity.
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