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Investigation on the Enrichment of DNA Using Lanthanum Carbonate
Author(s): KANG Xiaoyan, HE Anqi, WANG Jingdan, GUO Ran, ZHAI Yanjun, XU Yizhuang, WU Jinguang, College of Pharmacy, Liaoning University of Traditional Chinese Medicine, College of Chemistry and Molecular Engineering, Peking University, College of Chemistry and Material Science, Hebei Normal University
Pages: 7-
11
Year: 2016
Issue:
1
Journal: Chemical Journal of Chinese Universities
Keyword: Deoxyribonucleic acid(DNA); Lanthanum carbonate; Enrichment; Desorption;
Abstract: The adsorption behavior of Deoxyribonucleic acid( DNA) on lanthanum carbonate particles was investigated. Both UV and XPS results demonstrated that DNA could be adsorbed on the surface of lanthanum carbonate particles. Moreover,the efficiency of adsorption was maximized when the p H value of the solution was set around 5. 5. In addition,DNA could be desorpted when lanthanum carbonate particles with adsorpted DNA was dispersed in aqueous solution of KH2PO4. The ICP results demonstrated that the concentration of La3 +was lower than 0. 1 μmol / L in the aqueous solution after desorption process,thereby excluding the possibility of undesirable influence on DNA.
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