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Purification Process Optimization of Camellia Saponin by Response Surface Methodology
Author(s): 
Pages: 78-84
Year: Issue:  12
Journal: Cereals and Oils Processing

Keyword:  response surface methodologysaponinflocculation-precipitationrelease;
Abstract: In this paper, saponin was purified by flocculation-precipitation, and crude extraction from camellia oleifera seed shell was as raw material. On the basis of single factor experiments, purification process was optimized using response surface methodology, and a mathematical regression model was established by Mintab15.0 software. Optimal purification conditions obtained from this model were as follows: 16% Chitosan solution added in crude extraction; 1.78% Ca O was added in supernatant after flocculation to subside for 3.01 h at 32.75℃; 4.09% NH4HCO3 was added to the sediment, and then released at 52.2℃ for3.17 h. Under conditions, content of camellia saponin was 60.4% in the final.
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