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Study on Improvement of Chemosensitivity of MCF-7 Cells to Epirubicin and Inhibition of Aurora Kinase A in Treatment of Breast Cancer by San-Huang Decoction
Author(s): 
Pages: 2060-2068
Year: Issue:  10
Journal: World Science and Technology-Modernization of Traditional Chinese Medicine

Keyword:  San-Huang DecoctionAurora Kinase AMCF-7 cellscell proliferationapoptosis;
Abstract: This article was aimed to explore the effect of San-Huang(SH) decoction on improving chemosensitivity of MCF-7 cells to epirubicin and inhibition of Aurora kinase A, in order to discuss its underlying mechanism. The inhibition of MCF-7 cells proliferation on breast cancer by SH decoction was determined by CCK-8 assay. RT-PCR and western blot were used to detect the Aurora A, p53 m RNA and protein expression level of MCF-7 cells by SH decoction. The si RNA silenced Aurora A of MCF-7 cells. CCK-8 assay was used to detect the inhibition of MCF-7 cells proliferation. CCK-8 assay and Annexin V-FITC/PI staining were used to detect the inhibition rate and apoptosis rate of MCF-7 cells treated by the combination of SH decoction and epirubicin. Western blot analysis was used to detect the expression of apoptosis-related proteins. The results showed that SH decoction inhibited the proliferation of MCF-7 cells in a dose-dependent manner(P < 0.05). The effect of 48 h medication was better than 24 h(P < 0.05). There was no statistical difference with medication for 72 h(P > 0.05). SH decoction can regulate the Aurora A, p53 protein and m RNA expression of MCF-7 cells. si RNA silenced Aurora A, which downregulated the inhibition rate of MCF-7 cells by SH decoction for 50.0%(from 49.2% to 24.8%). The combination of SH decoction and epirubicin enhanced the effect of epirubicin on inhibiting the proliferation rate and apoptosis rate of MCF-7 cell, regulated the expression levels of apoptosis-related protein such as c-PARP, c-Caspase 3, Bcl-2, Bax, as well as the protein level of Aurora A. It was concluded that SH decoction can increase the chemosensitivity of MCF-7 cells to epirubicin, which may be related to the inhibition of Aurora Kinase A by SH decoction.
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