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Changes of Autophagy Activity in Carotid and Femoral Arteries of Tail Suspension Rats
Pages: 445-449
Year: Issue:  6
Journal: Space Medicine & Medical Engineering

Keyword:  simulated weightlessnessautophagycarotid arteryfemoral artery;
Abstract: Objective To investigate the changes of autophagy activity in the carotid arteries and femoral arteries of rats with tail suspension. Methods Male Sprague-Dawley rats were randomly divided into control and simulated weightlessness groups. Tail suspension for 28 d was used to simulate microgravity in this study. The expression of Microtubules-associated protein 1A light chain 3( LC3) and Bcl-2 interacting protein( Beclin-1)were detected by Western blot and RT-PCR in all groups. Moreover,immunohistochemistry assay were performed to evaluate the source of autophagy changes in vascular. Results Compared with the control group,the expression of mRNA and protein of Beclin 1 and LC3 had no significant change in the carotid artery of the simulated microgravity group,while Beclin 1 and LC3 expressions were significantly enhanced in the femoral arteries. Moreover,immunohistochemistry result showed that the expression of LC3 was increased in the endothelial layer and smooth muscle layer of the femoral artery. Conclusion The autophagy activity in the femoral arteries of rats under simulated weightlessness,tail suspension,for 28 d is stimulated,but not in the carotid artery.
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