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Protection of ginsenoside Rg3 on oxidative stress injury of human glomerular mesangial cells induced by H2O2 and its mechanism
Author(s): 
Pages: 1437-1442
Year: Issue:  12
Journal: World Phytomedicines

Keyword:  ginsenoside Rg 3human glomerular mesangial cellsoxidative stressprotective effect;
Abstract: Objective To investigate protective effects of ginsenoside Rg3 on oxidative stress injury of human glomerular mesangial cells induced by H2O2 and to explore its mechanism. Methods Oxidative stress injury models on human glomerular mesangial cells induced by H2O2 were established. Human glomerular mesangial cells were pretreated for 24 h by various concentrations of ginsenoside Rg3, then activity of cells, activity of LDH, and contents of MAD in culture medium were detected by CCK-8 test, LDH kit, and MDA kit. Cell cycle, m RNA level and protein express of CDK4 were determined by flow cytometry, q RT-PCR, and Western blotting. Results Stable and effective models of oxidative stress injury of human glomerular mesangial cells were established by 400 μmol/L H2O2 cultured for 24 h. With the increase of concentrations of ginsenoside Rg3, the amount of apoptotic cells damaged by H2O2 reduced, activity of LDH, and contents of MAD in culture medium were increased. Ginsenoside Rg3 with concentration of 40 μmol/L could block cell cycle in G1 phase, and down-regulate m RNA and protein expresses of CDK4 of human glomerular mesangial cells. Conclusion Ginsenoside Rg3 has protective effect on glomerular mesangial cells against oxidative stress injury of human glomerular mesangial cells induced by H2O2 which may be related to reduction of CDK4.
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