Author(s): GUO Song, LING Feng, WANG Jin-na, WU Yu-yan, HOU Juan, GONG Zhen-yu, Zhejiang Center for Disease Control and Prevention
Pages: 545-549
Year: 2015 Issue:  6
Journal: Chinese Journal of Vector Biology and Control
Keyword:  Aedes albopictus; Symbiotic bacteria; Wolbachia; wsp gene;
Abstract: Objective To investigate the infection state and genotype of Wolbachia from Aedes albopictus in central and eastern Zhejiang province, and to provide a reference for mosquito and mosquito-borne disease control.Methods Light traps and human baits were applied to collect adult female mosquitoes, wsp genes of Wolbachia were tested by PCR and sequenced for phylogenetic analysis.Results In total 192 adult female mosquitoes were captured in six sampling sites:Hangzhou, Ningbo, Shengsi, Yiwu, Lishui and Wenzhou. The positive rate of wsp gene of Wolbachia was between 25.0%and 96.9% among these cities. There were 11 wsp haplotype sequences, of which lengths were between 581 bp and 605 bp,the average content of A, T, G and C was 31.4%, 31.1%, 21.5%, 16.0%, respectively. The homology of the nucleotide sequences of wsp was between 76.5% and 99.8%, and the homology of the amino acid sequences of wsp was between 66.2%and 100%. The 11 wsp haplotype sequences were divided in super group A(Wolbachia: w Alb A) and B(Wolbachia: w Alb B)by phylogenetic analysis.Conclusion Infection of Wolbachia in Ae. albopictus mosquitoes are widespread by in centraland eastern Zhejiang province, whose genotypes belong to w AlbA and w AlbB.