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Effect of antimicrobial peptides LL37 combined with peptidoglycan on the differentiation of monocytes
Pages: 654-658
Year: Issue:  11
Journal: China journal of Leprosy and Skin Diseases

Keyword:  psoriasisantimicrobial peptides LL37peptidoglycanmonocytes;
Abstract: Objective:To determine the effect of antimicrobial peptides LL37 combined with peptidoglycan ( PGN) on the differentiation of monocytes. Methods:Monocytes from peripheral blood of healthy adults were cultured in the medium of LL37, PGN and LL37 combined PGN. The levels of triggering receptor expressed on myeloid cells-1( TREM-1) , CD14 and CD16 were detected by flow cytometry ( FCM) after 16 h culture. The levels of HLA-DR, CD1a and CD86 on immature dendritic cell ( iDC) were detected by FCM after culture for 5 days. T cells proliferation and Th17 polarization were detected by MTT and FCM respectively after iDC co-cultured with lymphocyte for 3 days. Results:The percentage of CD14++CD16+monocytes and Th17, the lev-els of TREM-1 and iDC and the proliferation of T cells in the LL37 combined PGN group were obviously high-er than those in the PGN group and LL37 group (P<0.05). Conclusion: Antimicrobial peptides LL37 in combination with PGN can induce the monocytes differentiation into CD14++CD16+, which finally induce the proliferation of T cells and differentiation to Th17.
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