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Genetic transformation and functional analysis of the SAMT gene in Chimonanthus praecox
Pages: 29-34
Year: Issue:  6
Journal: Journal of Nanjing Forestry University(Natural Science Edition)

Keyword:  Chimonanthus praecoxCpSAMTgenetic transformationPetunia hybridafloral scent metabolic puthwayGC-MS;
Abstract: In order to investigate the biological function of the Chimonanthus praecox SAMT gene, here we reported that the plant binary expression vector pBI121-CpSAMT was constructed and then the CpSAMT gene was introduced into pe?tunia through Agrobacterium tumefaciens mediated transformation. PCR and RT-PCR was used to test the transgenic plants, and HS-SPME-GC-MS was employed to analyze the components of flower flavor. Results showed that nine inde?pendent transgenic lines obtained were positive. Further analysis showed that transgenic plants were indistinguishable from untransformed controls in terms of plant size, leaf morphology, flower color, petal size and flowering time. The a?nalysis of HS-SPME-GC-MS showed that the content of benzaldehyde, phenylethanol, citronellol, citronellyl acetate and phenethyl acetate in flowers of transgenic CpSAMT plants was increased. However, the contents of methyl salicylate and methyl benzoate had not been significantly changed comparing with non?transgenic controls.
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