The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit later.
We apologize for any inconvenience caused
Login  | Sign Up  |  Oriprobe Inc. Feed
China/Asia On Demand
Journal Articles
Laws/Policies/Regulations
Companies/Products
Bookmark and Share
Isolation and identification of Japanese encephalitis virus from swine
Author(s): 
Pages: 1698-1703
Year: Issue:  10
Journal: Acta Agriculturae Zhejiangensis

Keyword:  Japanese encephalitis virusisolation and identificationbiological characteristics;
Abstract: 从江苏某发病猪场采集的母猪流产胎儿脑组织样本中成功分离到一株猪源乙型脑炎病毒(JEV)毒株,并对该毒株的相关生物学特性进行了鉴定.利用BHK-21细胞传代和脑内接种小鼠方法,对疑似阳性病料做病毒分离.通过RT-PCR,CPE和IFA等方法对JEV分离株进行鉴定,并测定其TCID50.结果成功分离获得一株JEV毒株(JEV JS01株),该毒株能够在BHK-21细胞上增殖并产生细胞病变,TCID50为每1 mL10 7 0,免疫荧光检测可观察到JEV特异性荧光.RT-PCR扩增E基因并测序分析表明,与GenBank中登录的JEV毒株序列具有较高的同源性,属基因Ⅲ型.小鼠致病力试验表明该分离株对易感动物具有较高致病性.本研究为进一步开展乙脑病毒流行病学与疫苗免疫研究奠定了基础.
Related Articles
No related articles found