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rDNA sequence analysis and morphological redescription of Empoasca onukii from the tea growing area of Fujian
Pages: 1773-1780
Year: Issue:  10
Journal: Acta Agriculturae Zhejiangensis

Keyword:  Empoasca onukiimorphology;
Abstract: 重新描述了小贯小绿叶蝉的形态结构,尤其是头部色斑、翅脉、腹部内突、下生殖板及其刚毛着生位置,并增加了足部(刺毛列)特征描述.通过PCR技术克隆得到该物种的rDNA序列,包括部分18S(1 843bp)和28S序列(667 bp)以及完整的5.8S(155 bp)、ITS1(3 114 bp)和ITS2序列(1 008 bp).序列分析表明,18S,5.8S,28S与其他物种间具有90%~ 95%的序列一致性;而ITS1和ITS2序列变异性非常大.碱基组成比率分析显示ITS1以及ITS2具有AT偏好性,前者A+T占66.0%,后者占65.1%.与亲缘种的比较分析显示ITS1和ITS2具有丰富的多态位点,并且ITS2更适用于近缘种的分子鉴定.
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