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Identification of a DMP-degraded strain in black soil and the optimation of degradation conditions
Author(s): 
Pages: 1807-1812
Year: Issue:  10
Journal: Acta Agriculturae Zhejiangensis

Keyword:  dimethyl phthalateblack soilPAEs pollutiondegradation bacteriaorthogonal optimize test;
Abstract: 邻苯二甲酸二甲酯(DMP)作为一种在环境中广泛存在的有毒化合物,已被中国列为优先控制污染物.采用无机盐培养基从长期覆盖农膜的黑土中分离鉴定了一株能够以DMP为碳源的菌株QD-9-10,根据菌落的形态特征及16S rDNA碱基序列同源性分析,鉴定QD-9-10为Bacillus sp..正交实验结果表明,菌株QD-9-10的最优降解条件为pH8.0,温度35.0℃,摇床转速124.0 r·min-1,DMP初始浓度100.4 mg·L-1,在32 h时内可将DMP完全降解.底物利用能力测试表明,菌种QD-9-10具有可降解多种邻苯二甲酸酯(PAE)的能力.由此可见,Bacillus sp.QD-9-10在修复PAEs污染环境方面有一定应用前景.
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